Genome-Wide Association Study Reveals Novel Quantitative Trait Loci Associated with Resistance to Multiple Leaf Spot Diseases of Spring Wheat

被引:147
作者
Gurung, Suraj [1 ]
Mamidi, Sujan [2 ]
Bonman, J. Michael [3 ]
Xiong, Mai [4 ]
Brown-Guedira, Gina [4 ]
Adhikari, Tika B. [5 ,6 ]
机构
[1] Univ Calif Davis, Dept Plant Pathol, USDA ARS, Salinas, CA USA
[2] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA
[3] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID USA
[4] N Carolina State Univ, USDA ARS, Dept Crop Sci, Plant Sci Res Unit, Raleigh, NC 27695 USA
[5] N Carolina State Univ, Ctr Integrated Pest Management, Raleigh, NC 27695 USA
[6] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA
来源
PLOS ONE | 2014年 / 9卷 / 09期
基金
美国食品与农业研究所;
关键词
SEPTORIA-TRITICI BLOTCH; STAGONOSPORA-NODORUM BLOTCH; FUSARIUM HEAD BLIGHT; HOST-SELECTIVE TOXIN; PATHOGEN-INDUCED EXPRESSION; IRON-DEFICIENCY CHLOROSIS; TAN SPOT; LINKAGE DISEQUILIBRIUM; ARABIDOPSIS-THALIANA; POPULATION-STRUCTURE;
D O I
10.1371/journal.pone.0108179
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Accelerated wheat development and deployment of high-yielding, climate resilient, and disease resistant cultivars can contribute to enhanced food security and sustainable intensification. To facilitate gene discovery, we assembled an association mapping panel of 528 spring wheat landraces of diverse geographic origin for a genome-wide association study (GWAS). All accessions were genotyped using an Illumina Infinium 9K wheat single nucleotide polymorphism (SNP) chip and 4781 polymorphic SNPs were used for analysis. To identify loci underlying resistance to the major leaf spot diseases and to better understand the genomic patterns, we quantified population structure, allelic diversity, and linkage disequilibrium. Our results showed 32 loci were significantly associated with resistance to the major leaf spot diseases. Further analysis identified QTL effective against major leaf spot diseases of wheat which appeared to be novel and others that were previously identified by association analysis using Diversity Arrays Technology (DArT) and bi-parental mapping. In addition, several identified SNPs co-localized with genes that have been implicated in plant disease resistance. Future work could aim to select the putative novel loci and pyramid them in locally adapted wheat cultivars to develop broad-spectrum resistance to multiple leaf spot diseases of wheat via marker-assisted selection (MAS).
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页数:20
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共 101 条
  • [1] Whole-Genome QTL Analysis of Stagonospora nodorum Blotch Resistance and Validation of the SnTox4-Snn4 Interaction in Hexaploid Wheat
    Abeysekara, Nilwala S.
    Faris, Justin D.
    Chao, Shiaoman
    McClean, Phillip E.
    Friesen, Timothy L.
    [J]. PHYTOPATHOLOGY, 2012, 102 (01) : 94 - 104
  • [2] Identification and characterization of a novel host-toxin interaction in the wheat-Stagonospora nodorum pathosystem
    Abeysekara, Nilwala S.
    Friesen, Timothy L.
    Keller, Beat
    Faris, Justin D.
    [J]. THEORETICAL AND APPLIED GENETICS, 2009, 120 (01) : 117 - 126
  • [3] Identification and molecular mapping of a gene in wheat conferring resistance to Mycosphaerella graminicola
    Adhikari, TB
    Anderson, JM
    Goodwin, SB
    [J]. PHYTOPATHOLOGY, 2003, 93 (09) : 1158 - 1164
  • [4] Molecular mapping of Stb1, a potentially durable gene for resistance to septoria tritici blotch in wheat
    Adhikari, TB
    Yang, X
    Cavaletto, JR
    Hu, X
    Buechley, G
    Ohm, HW
    Shaner, G
    Goodwin, SB
    [J]. THEORETICAL AND APPLIED GENETICS, 2004, 109 (05) : 944 - 953
  • [5] Molecular mapping of the Stb4 gene for resistance to Septoria tritici blotch in wheat
    Adhikari, TB
    Cavaletto, JR
    Dubcovsky, J
    Gieco, JO
    Schlatter, AR
    Goodwin, SB
    [J]. PHYTOPATHOLOGY, 2004, 94 (11) : 1198 - 1206
  • [6] Association Mapping of Quantitative Trait Loci in Spring Wheat Landraces Conferring Resistance to Bacterial Leaf Streak and Spot Blotch
    Adhikari, Tika B.
    Gurung, Suraj
    Hansen, Jana M.
    Jackson, Eric W.
    Bonman, J. Michael
    [J]. PLANT GENOME, 2012, 5 (01): : 1 - 16
  • [7] Association Mapping of Quantitative Resistance to Phaeosphaeria nodorum in Spring Wheat Landraces from the USDA National Small Grains Collection
    Adhikari, Tika B.
    Jackson, Eric W.
    Gurung, Suraj
    Hansen, Jana M.
    Bonman, J. Michael
    [J]. PHYTOPATHOLOGY, 2011, 101 (11) : 1301 - 1310
  • [8] Association mapping of yield and its components in rice cultivars
    Agrama, H. A.
    Eizenga, G. C.
    Yan, W.
    [J]. MOLECULAR BREEDING, 2007, 19 (04) : 341 - 356
  • [9] [Anonymous], 2013, FAO Statistical Yearbook 2013: World food and agriculture
  • [10] A gene in European wheat cultivars for resistance to an African isolate of Mycosphaerella graminicola
    Arraiano, L. S.
    Chartrain, L.
    Bossolini, E.
    Slatter, H. N.
    Keller, B.
    Brown, J. K. M.
    [J]. PLANT PATHOLOGY, 2007, 56 (01) : 73 - 78