Transcriptomic and metabolomic profiling of drought-tolerant and susceptible sesame genotypes in response to drought stress

被引：180

作者：

You, Jun ^{[1
]}

Zhang, Yujuan ^{[1
,2
]}

Liu, Aili ^{[1
]}

Li, Donghua ^{[1
]}

Wang, Xiao ^{[3
]}

Dossa, Komivi ^{[1
,4
]}

Zhou, Rong ^{[1
]}

Yu, Jingyin ^{[1
]}

Zhang, Yanxin ^{[1
]}

Wang, Linhai ^{[1
]}

Zhang, Xiurong ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Oil Crops Res Inst, Minist Agr & Rural Affairs, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Hubei, Peoples R China

[2] Shandon Acad Agr Sci, Shandong Cotton Res Ctr, Special Econ Crop Res Ctr, Jinan 250100, Shandong, Peoples R China

[3] Minist Agr & Rural Affairs, Qual Inspect & Test Ctr Oilseed Prod, Wuhan 430062, Hubei, Peoples R China

[4] CERAAS, Thies 3320, Senegal

来源：

BMC PLANT BIOLOGY | 2019年 / 19卷 / 1期

基金：

中国国家自然科学基金;

关键词：

Sesame; Drought stress; Transcriptome; Metabolome; Abscisic acid; Amino acids; OVER-EXPRESSION; ACID; ARABIDOPSIS; PATHWAY; LEAVES; SALT; PHOTOSYNTHESIS; METABOLITES; ANNOTATION; ACTIVATION;

D O I：

10.1186/s12870-019-1880-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

BackgroundSesame is an important oil crop due to its high oil, antioxidant, and protein content. Drought stress is a major abiotic stress that affects sesame production as well as the quality of sesame seed. To reveal the adaptive mechanism of sesame in response to water deficient conditions, transcriptomic and metabolomics were applied in drought-tolerant (DT) and drought-susceptible (DS) sesame genotypes.ResultsTranscriptomic analysis reveals a set of core drought-responsive genes (684 up-regulated and 1346 down-regulated) in sesame that was robustly differently expressed in both genotypes. Most enriched drought-responsive genes are mainly involved in protein processing in endoplasmic reticulum, plant hormone signal transduction photosynthesis, lipid metabolism, and amino acid metabolism. Drought-susceptible genotype was more disturbed by drought stress at both transcriptional and metabolic levels, since more drought-responsive genes/metabolites were identified in DS. Drought-responsive genes associated with stress response, amino acid metabolism, and reactive oxygen species scavenging were more enriched or activated in DT. According to the partial least-squares discriminate analysis, the most important metabolites which were accumulated under drought stress in both genotypes includes ABA, amino acids, and organic acids. Especially, higher levels of ABA, proline, arginine, lysine, aromatic and branched chain amino acids, GABA, saccharopine, 2-aminoadipate, and allantoin were found in DT under stress condition. Combination of transcriptomic and metabolomic analysis highlights the important role of amino acid metabolism (especially saccharopine pathway) and ABA metabolism and signaling pathway for drought tolerance in sesame.ConclusionThe results of the present study provide valuable information for better understanding the molecular mechanism underlying drought tolerance of sesame, and also provide useful clues for the genetic improvement of drought tolerance in sesame.

引用

页数：16

共 73 条

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