Determination of ammonium perfluorooctoate in the teflon coating of the non-stick pan using liquid chromatography-tandem mass spectrometry with accelerated solvent extraction

A novel and rapid method, based on liquid chromatography tandem mass spectrometry (LC-MS/MS) with accelerated solvent ex traction (ASE) was developed for quantitative detection of trace ammonium perfluorooctoate (APFO) in the Teflon coating of the non-stick pan. APFO residue was extracted from the Tef-Ion coating of the non-stick pan by acetonitrile with accelerated solvent extractor (10335 kPa, 175 degrees C, seven minutes' extration twice). The extract was determined by high performance liquid chromatography using acetonitrile-water (10 mmol/L ammonium acetate) as mobile phase. The APFO was detected using electrospay ionization (ESI) on a tandemn mass spectrometer in multiple reaction monitoring mode. The Linear calibration curve was obtained in the range of 0.5 mu g/L - 100 mu g/L with a linear correlation coefficient (R-2) of 0.999. The low detection limit (LDL) was 1 ng/g. The APFO recovery in the Telfon coating of the non-stick pan was ranged from 80.5% to 124.6% with the relative standard deviation (RSD) from 1.3%. to 9.3%. The detection method proposed was accurate, expedite, simple and convenient.