A new cultivation-independent tool for fast and reliable detection of Mycobacterium marinum

被引：6
作者：
Slany, M. [1 ]
机构：
[1] Vet Res Inst, Mycobacteriol Unit, OIE Reference Labs ParaTB & Avian TB, Brno 62100, Czech Republic
来源：
JOURNAL OF FISH DISEASES | 2014年 / 37卷 / 04期
关键词：
molecular microbiology; fish mycobacteriosis; real-time PCR; bacterial typing; cutaneous infection; zoonosis; REAL-TIME PCR; ULCERANS; MYCOLACTONE; INFECTIONS; VIRULENCE; SAMPLES; ERP;
D O I：
10.1111/jfd.12113
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
The Mycobacterium marinum group (MMG) is a class of mycobacteria that includes M.marinum, the cause of chronic systemic infections in fish. This species occasionally causes granulomatous skin lesions in humans. Other members of MMG are mycolactone-producing mycobacteria (MPM; M.ulcerans, M.shottsii and M.pseudoshottsii). The cultivation-independent approach presented in this study brings a fast and reliable alternative to classically used cultivation methods. The developed triplex erp/IS2404 qPCR assay is based on a primary species-specific erp detection, which allows enumeration of MMG in analysed samples, and secondary IS2404 detection is suitable for the differentiation of M.marinum from MPM. The detection of M.marinum in clinical specimens and in artificially contaminated tissue samples has proven its applicability for diagnostic purposes.
引用
收藏
页码：363 / 369
页数：7
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