In vitro studies on the structure and properties of silk fibroin aqueous solutions in silkworm

被引：21

作者：

Jin, Yuan ^{[1
]}

Hang, Yichun ^{[1
]}

Luo, Jie ^{[1
]}

Zhang, Yaopeng ^{[1
]}

Shao, Huili ^{[1
]}

Hu, Xuechao ^{[1
]}

机构：

[1] Donghua Univ, Coll Mat Sci & Engn, State Key Lab Modificat Chem Fibers & Polymer Mat, Shanghai 201620, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2013年 / 62卷

基金：

中国国家自然科学基金;

关键词：

Silk fibroin; Birefringence; Conformation; Rheology; Aggregates; CONFORMATION TRANSITION; LIQUID; PH;

D O I：

10.1016/j.ijbiomac.2013.08.027

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The spinning process of silkworm in vivo attracts great attentions. In this work, the structures and properties of the silk fibroin (SF) aqueous solutions from different divisions of silk glands of silkworms were investigated by using polarized microscope, rotational rheometer, Raman spectrometer and dynamic laser light scattering instrument. It was found that only the anterior (A) division and the anterior part of middle division (MA) of silk gland showed birefringence. With flowing from the posterior part (MP) to the MA part in the middle division of silk gland, the SF aqueous solutions was gradually transformed from random coil/alpha-helix to beta-sheet conformation. Meantime, the elastic and viscous nature of the SF aqueous solution changed, and the mean diameter of SF aggregates increased from 118 nm to 331 nm. It could be concluded that the structures and properties of the SF aqueous solutions were gradually changed along the silk gland and the liquid crystal structure was initially formed in the MA part of silk gland. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：162 / 166

页数：5

共 25 条

[1] Role of pH and charge on silk protein assembly in insects and spiders [J].