Development of new-tools to investigate toxicological hazard due to endocrine disruptor organochlorines and emerging contaminants in Mediterranean cetaceans

被引：22

作者：

Fossi, M. Cristina ^{[1
]}

Marsili, Letizia ^{[1
]}

Casini, Silvia ^{[1
]}

Bucalossi, Daniela ^{[1
]}

机构：

[1] Univ Siena, Dept Environm Sci, I-53100 Siena, Italy

来源：

MARINE ENVIRONMENTAL RESEARCH | 2006年 / 62卷 / S200-S204期

关键词：

Mediterranean cetaceans; organochlorine contaminants; brominated flame retardants; endocrine disrupting chemicals; fibroblasts cell culture; immunofluorescence technique; western blot;

D O I：

10.1016/j.marenvres.2006.04.063

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The possibility that certain Mediterranean cetaceans are subject to toxicological hazard due to organochlorines and emerging contaminants, such as polybrominated diphenyl ethers (PBDEs) with endocrine disrupting capacity, was investigated using non-lethal methods. The need for new biomarkers for EDCs and for a "cell model" to explore the different susceptibilities to several classes of ECDs, including emerging contaminants, led us to culture fibroblasts of different cetacean species ("dolphins in test tubes"). We then explored interspecies and gender susceptibility to OGEDCs and PBDEs using qualitative and semi-quantitative evaluation of target proteins, such as CYP1A and CYP2B in cultured cetacean fibroblasts (Stenella coeruleoalba, Tursiops truncatus and Balaenoptera physalus), by western blot and immunofluorescence techniques. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：S200 / S204

页数：5

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