EuPIP1;2, a Plasma Membrane Aquaporin Gene from Eucommia ulmoides, Enhances Drought and Salt Tolerance in Transgenic Tobacco

被引:7
|
作者
Chen, Jiajia [1 ]
Li, Jianrong [1 ]
Huang, Yanhui [1 ]
Li, Yan [1 ]
Su, Changfeng [1 ]
Zeng, Xiaofang [1 ]
机构
[1] Guizhou Univ, Coll Life Sci, Inst Agrobioengn,Minist Educ, Key Lab Plant Resource Conservat & Germplasm Inno, Guiyang 550025, Peoples R China
来源
AGRONOMY-BASEL | 2022年 / 12卷 / 03期
关键词
EuPIP1; 2; drought stress; salt stress; resistance; F INERME MAKINO; ARABIDOPSIS-THALIANA; STRESS; EXPRESSION; RESPONSES;
D O I
10.3390/agronomy12030615
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Aquaporins are a specific type of membrane channel proteins that efficiently transport water molecules and other small molecular substrates in plants. In this study, we isolated the plasma membrane aquaporin gene EuPIP1;2 from Eucommia ulmoides, an important medicinal plant in China. The EuPIP1;2 protein was localized on the plasma membrane. Quantitative RT-PCR analysis revealed that EuPIP1;2 was constitutively expressed in all analyzed tissues, with the highest expression levels detected in the fruit and root. Overexpression of EuPIP1;2 in transgenic tobacco enhanced plant tolerance of drought and salinity. Under drought and salt stress, the transgenic lines exhibited higher percentage germination, longer roots, and enhanced percentage survival compared with wild-type plants. The contents of malonaldehyde and proline suggested that EuPIP1;2 improved drought and salt tolerance in transgenic lines by reducing damage to membranes and improving osmotic adjustment. We predict that EuPIP1;2 could be applied to improve drought and salt tolerance in transgenic plants.
引用
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页数:14
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