EuPIP1;2, a Plasma Membrane Aquaporin Gene from Eucommia ulmoides, Enhances Drought and Salt Tolerance in Transgenic Tobacco

被引：9

作者：

Chen, Jiajia ^{[1
]}

Li, Jianrong ^{[1
]}

Huang, Yanhui ^{[1
]}

Li, Yan ^{[1
]}

Su, Changfeng ^{[1
]}

Zeng, Xiaofang ^{[1
]}

机构：

[1] Guizhou Univ, Coll Life Sci, Inst Agrobioengn,Minist Educ, Key Lab Plant Resource Conservat & Germplasm Inno, Guiyang 550025, Peoples R China

来源：

AGRONOMY-BASEL | 2022年 / 12卷 / 03期

关键词：

EuPIP1; 2; drought stress; salt stress; resistance; F INERME MAKINO; ARABIDOPSIS-THALIANA; STRESS; EXPRESSION; RESPONSES;

D O I：

10.3390/agronomy12030615

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Aquaporins are a specific type of membrane channel proteins that efficiently transport water molecules and other small molecular substrates in plants. In this study, we isolated the plasma membrane aquaporin gene EuPIP1;2 from Eucommia ulmoides, an important medicinal plant in China. The EuPIP1;2 protein was localized on the plasma membrane. Quantitative RT-PCR analysis revealed that EuPIP1;2 was constitutively expressed in all analyzed tissues, with the highest expression levels detected in the fruit and root. Overexpression of EuPIP1;2 in transgenic tobacco enhanced plant tolerance of drought and salinity. Under drought and salt stress, the transgenic lines exhibited higher percentage germination, longer roots, and enhanced percentage survival compared with wild-type plants. The contents of malonaldehyde and proline suggested that EuPIP1;2 improved drought and salt tolerance in transgenic lines by reducing damage to membranes and improving osmotic adjustment. We predict that EuPIP1;2 could be applied to improve drought and salt tolerance in transgenic plants.

引用

页数：14

共 43 条

[1] The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis [J].