Bioactive peptide of Cicer arietinum L. induces apoptosis in human endometrial cancer via DNA fragmentation and cell cycle arrest

被引：15

作者：

Gupta, Neha ^{[1
]}

Bhagyawant, Sameer Suresh ^{[1
]}

机构：

[1] Jiwaji Univ, Sch Studies Biotechnol, Gwalior 474011, Madhya Pradesh, India

来源：

3 BIOTECH | 2021年 / 11卷 / 02期

关键词：

Alcalase; Bioactive; Chickpea peptide; Human cancer cells;

D O I：

10.1007/s13205-020-02614-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Chickpea seed proteins are alleged source of nutraceuticals. These seed proteins were subjected to different proteases to produce peptides. The efficacy of these peptides was confirmed using six diverse human cancer cell lines (PA-1, Ishikawa cells, A549, MCF-7, HepG2, MDA-MB-231). Alcalase generated peptides exhibited the highest antagonistic inhibition of Ishikawa cells. Flow cytometric analysis revealed that chickpea peptide induced S and G2 phase arrest of cell cycle in a dose dependent manner. DNA fragmentation and apoptosis occurred by down regulation of Bcl-2 expression, upregulation of Bax expression and promotion of caspase-3 initiation. Chickpea peptides ascertain potential antiproliferative molecule that can be deployed in cancer treatment regimes.

引用

页数：13

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