Antiproliferative Effect of the Methanol Extract of Piper crocatum Ruiz & Pav Leaves on Human Breast (T47D) Cells In-vitro

被引：0

作者：

Wicaksono, Britanto Dani ^{[1
]}

Handoko, Yohana Ayupriyanti ^{[2
,3
]}

Arung, Enos Tangke ^{[1
,2
,3
]}

Kusuma, Irawan Wijaya ^{[2
,3
]}

Yulia, Dina ^{[1
]}

Pancaputra, Aldrin Neilwan ^{[4
]}

Sandra, Ferry ^{[1
]}

机构：

[1] Stem Cell & Canc Inst, Jakarta 13210, Indonesia

[2] Mulawarman Univ, Fac Forestry, Dept Forest Prod, Wood Chem Lab, Kelua, Indonesia

[3] Jalan KH Dewantara, Samarinda 75123, E Borneo, Indonesia

[4] Dharmasis Canc Hosp, Jakarta 11420, Indonesia

来源：

TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH | 2009年 / 8卷 / 04期

关键词：

Piper crocatum leaf; methanol extract; antiproliferative; breast cancer; T47D cells; PLANT ANTITUMOR AGENTS; FLAVONOIDS;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Purpose: To investigate the anti-cancer properties of the methanol extract of Piper crocatum Ruiz & Pav. leaves and its mode of action in human breast cancer (T47D) cells. Methods: The anti-cancer property and mechanism of action of the extract were evaluated by its effect on cell viability, nuclear morphology, cell cycle progression and the expression of phosphorylated p44/p42 as a marker for cell proliferation. Results: The results showed that there was a reduction of cell viability by the extract in a concentration-dependent manner and no alteration of nuclear morphology was observed. There were negligible changes in Sub-G1 phase formation after extract treatment. Expression of phosphorylated p44/42 was decreased by the extract only. Inclusion of the extract in the incubation medium decreased insulin-stimulated phosphorylation of p44/p42 indicating that the anti-proliferative effect of the extract was via p44/p42 pathway. Conclusion: All together, the data indicate that P. crocatum methanol extract inhibits the growth of human breast cancer (T47D) cells via inhibition of p44/p42 phosphorylation.

引用

页码：345 / 352

页数：8

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