Proteins and antibodies in serum, plasma, and whole blood-size characterization using asymmetrical flow field-flow fractionation (AF4)

被引：163

作者：

Leeman, Mats ^{[1
]}

Choi, Jaeyeong ^{[2
]}

Hansson, Sebastian ^{[1
]}

Storm, Matilda Ulmius ^{[1
]}

Nilsson, Lars ^{[2
]}

机构：

[1] SOLVE Res & Consultancy AB, S-22381 Lund, Sweden

[2] Lund Univ, Fac Engn LTH, Dept Food Technol Engn & Nutr, S-22100 Lund, Sweden

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2018年 / 410卷 / 20期

关键词：

Whole blood; Antibodies; Plasma; Serum; Asymmetric flow field-flow fractionation (AF4); Fluorescence labelling; ORTHOGONAL METHODS; SEPARATION; QUANTITATION; MACROMOLECULES; IMMUNOGENICITY; CHROMATOGRAPHY;

D O I：

10.1007/s00216-018-1127-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The analysis of aggregates of therapeutic proteins is crucial in order to ensure efficacy and patient safety. Typically, the analysis is performed in the finished formulation to ensure that aggregates are not present. An important question is, however, what happens to therapeutic proteins, with regard to oligomerization and aggregation, after they have been administrated (i.e., in the blood). In this paper, the separation of whole blood, plasma, and serum is shown using asymmetric flow field-flow fractionation (AF4) with a minimum of sample pre-treatment. Furthermore, the analysis and size characterization of a fluorescent antibody in blood plasma using AF4 are demonstrated. The results show the suitability and strength of AF4 for blood analysis and open new important routes for the analysis and characterization of therapeutic proteins in the blood.

引用

页码：4867 / 4873

页数：7

共 29 条

[1]

Abbas A.K., 2000, Cellular and Molecular Immunology, V4th

[2]

Barrett KE, 2010, GANONGS REV MED PHYS

[3] The future of protein particle characterization and understanding its potential to diminish the immunogenicity of biopharmaceuticals: A shared perspective [J].