Hybridization of Aegilops caudata with Triticum durum, Triticum aestivum and the detection of alien chromatins

Aegilops caudata L. carries resistance genes against wheat diseases as well as genes of high crude protein and lysine contents, which can be useful for wheat improvement. An amphiploid of Triticum durum - Ae. caudata was synthesized and the hybridization of T. aestivum with the amphiploid Am 8 was carried out. Chromosome in situ hybridization was carried out for the PMC (pollen mother cell) of the synthesized amphiploid (AABBCC) and (T. aestivum Beijing 837 x Am 8) F-2 by using the pAeca 212 as a probe. The results showed that the 7 bivalents from C genome had hybridization signals in the amphiploid. The detection for F-2 (Beijing 837 x Am 8) indicated that translocation, even (pure) home translocation, occurred in F-2 generations spontaneously. The study showed the bright prospect in transferring alien resistance genes from C genome to wheat.