Stoichiometry of phage T4 Dam DNA(adenine-N6)-methyltransferase binding with oligonucleotide substrates

被引：0

作者：

Zinovev, VV

Ovechkina, LG

Malygin, EG

机构：

来源：

MOLECULAR BIOLOGY | 1996年 / 30卷 / 05期

关键词：

DNA methyltransferase; oligonucleotides; binding stoichiometry;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phage T4 Dam DNA methyltransferase recognizes the GATC site and methylates the adenine N6. The stoichiometry of its complex with the substrate was assessed by gel filtration and sucrose gradient ultracentrifugation. The results obtained by both methods indicate that two enzyme subunits bind with 32- and 20-bp DNA duplexes.

引用

页码：724 / 726

页数：3

共 14 条

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