Comparison of capsular antigens of Pasteurella multocida strains

被引:0
作者
Das, SC [1 ]
Mishra, S [1 ]
Mishra, N [1 ]
Bhagwan, PSK [1 ]
机构
[1] Cent Sheep & Wool Res Inst, Avikanagar 304501, Rajasthan, India
关键词
capsular antigens; Pasteurella multocida;
D O I
暂无
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The present study was undertaken to compare capsular antigens of Pasteurella multocida strains isolated from a field case of ovine pneumania (type B) with a reference strain. Capsular antigens were extracted by saline solution, potassium thiocyanate and sodium salicylate solution separately. Our of these 3 methods, sodium chloride extraction method resulted in the highest protein concentration in both the strains as compared to other methods. Protein profile of both the strains by SDS- PAGE revealed more number of bands by the same method of extraction. Saline solution extract was able to produce high antibody titre, whereas sodium salicylate extract was more cross-reactive.
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页码:872 / 874
页数:3
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