Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi

被引:12
作者
Agusti, R
Couto, AS
Alves, MJM
Colli, W [1 ]
de Lederkremer, RM
机构
[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Organ, Cihidecar, RA-1428 Buenos Aires, DF, Argentina
[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05599970 Sao Paulo, Brazil
来源
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2000年 / 95卷 / 01期
关键词
C-14]-ethanolamine and [H-3]-palmitic acid incorporation; phospholipids; desaturated fatty acids;
D O I
10.1590/S0074-02762000000100016
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Trypomastigote forms of Trypanosoma cruzi were metabolically, labeled with [C-14]-ethanolamine and [H-3]-palmitic acid. Lipids shed to the culture medium were analyzed and compared with the parasite components. Phosphatidylcholine and lysophosphatidylcholine accounted for 53% of the total incorporated precursor. interestingly, phosphatidylethanolamine and its lyse derivative lysophosphatidylethanolamine, although present in significant amounts in the parasites, could not be detected in the shed material. Shed lipids were highly enriched in the desaturated fatty acids C-16:1 and C-18:1 when compared to the total fatty acid pool isolated from the parasites.
引用
收藏
页码:97 / 102
页数:6
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