Ophiopogonin D Increases SERCA2a Interaction with Phospholamban by Promoting CYP2J3 Upregulation

被引：11
作者：
Wang, Jia [1 ,2 ]
You, Wenting [1 ,3 ]
Wang, Ningning [1 ,2 ]
Zhou, Wei [1 ]
Ge, Yunxuan [1 ]
Ma, Zengchun [1 ]
Tan, Hongling [1 ]
Wang, Yuguang [1 ]
Gao, Yue [1 ]
机构：
[1] Beijing Inst Radiat Med, Dept Pharmaceut Sci, Beijing 100850, Peoples R China
[2] Guangdong Pharmaceut Univ, Guangzhou 510006, Peoples R China
[3] Anhui Med Univ, Dept Pharmacol, Hefei 230032, Peoples R China
来源：
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY | 2020年 / 2020卷
基金：
美国国家科学基金会;
关键词：
DEPENDENT PROTEIN-KINASE; EPOXYEICOSATRIENOIC ACIDS; RAT CARDIOMYOCYTES; TRANSCRIPTIONAL REGULATION; ATPASE INTERACTION; HEART-FAILURE; GENE-THERAPY; ACTIVATION; HOMEOSTASIS; MODULATION;
D O I：
10.1155/2020/8857906
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Ophiopogonin D (OPD), a compound from the Chinese herb Radix Ophiopogonis, reportedly induces increased levels of cytochrome P450 2J3 (CYP2J3)/epoxyeicosatrienoic acids (EETs) and Ca2+ in rat cardiomyocytes. Little is known regarding the specific mechanism between CYP2J3 and Ca2+ homeostasis. Here, we investigated whether CYP2J3 is involved in the protective action of OPD on the myocardium by activating the Ca2+ homeostasis-related protein complex (SERCA2a and PLB) in H9c2 rat cardiomyoblast cells. The interaction between SERCA2a and PLB was measured using fluorescence resonance energy transfer. OPD attenuated heart failure and catalyzed the active transport of Ca2+ into the sarcoplasmic reticulum by inducing the phosphorylation of PLB and promoting the SERCA2a activity. These beneficial effects of OPD on heart failure were abolished after knockdown of CYP2J3 in a model of heart failure. Together, our results identify CYP2J3 as a critical intracellular target for OPD and unravel a mechanism of CYP2J3-dependent regulation of intracellular Ca2+.
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页数：22
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共 40 条
[1]   PPARα agonists inhibit inflammatory activation of macrophages through upregulation of β-defensin 1 [J].
Ann, Soo-jin ;
Chung, Ji Hyung ;
Park, Byung Hee ;
Kim, Soo Hyuk ;
Jang, Jiyoung ;
Park, Sungha ;
Kang, Seok-Min ;
Lee, Sang-Hak .
ATHEROSCLEROSIS, 2015, 240 (02) :389-397
[2]   New role for epoxyeicosatrienoic acids as anti-inflammatory mediators [J].
Campbell, WB .
TRENDS IN PHARMACOLOGICAL SCIENCES, 2000, 21 (04) :125-127
[3]   Targeting phospholamban by gene transfer in human heart failure [J].
del Monte, F ;
Harding, SE ;
Dec, GW ;
Gwathmey, JK ;
Hajjar, RJ .
CIRCULATION, 2002, 105 (08) :904-907
[4]   Calcium/Calmodulin-Dependent Protein Kinase II Activity Persists During Chronic -Adrenoceptor Blockade in Experimental and Human Heart Failure [J].
Dewenter, Matthias ;
Neef, Stefan ;
Vettel, Christiane ;
Laemmle, Simon ;
Beushausen, Christina ;
Zelarayan, Laura C. ;
Katz, Sylvia ;
von der Lieth, Albert ;
Meyer-Roxlau, Stefanie ;
Weber, Silvio ;
Wieland, Thomas ;
Sossalla, Samuel ;
Backs, Johannes ;
Brown, Joan H. ;
Maier, Lars S. ;
El-Armouche, Ali .
CIRCULATION-HEART FAILURE, 2017, 10 (05)
[5]   ALTERATIONS IN HEART MEMBRANE CALCIUM-TRANSPORT DURING THE DEVELOPMENT OF ISCHEMIA-REPERFUSION INJURY [J].
DHALLA, NS ;
PANAGIA, V ;
SINGAL, PK ;
MAKINO, N ;
DIXON, IMC ;
EYOLFSON, DA .
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1988, 20 :3-13
[6]   ER Stress Negatively Modulates the Expression of the miR-199a/214 Cluster to Regulates Tumor Survival and Progression in Human Hepatocellular Cancer [J].
Duan, Quanlu ;
Wang, Xingxu ;
Gong, Wei ;
Ni, Li ;
Chen, Chen ;
He, Xingxing ;
Chen, Fuqiong ;
Yang, Lei ;
Wang, Peihua ;
Wang, Dao Wen .
PLOS ONE, 2012, 7 (02)
[7]   Alterations in cytochrome P450-derived arachidonic acid metabolism during pressure overload-induced cardiac hypertrophy [J].
El-Sherbeni, Ahmed A. ;
El-Kadi, Ayman O. S. .
BIOCHEMICAL PHARMACOLOGY, 2014, 87 (03) :456-466
[8]   Fluorescence Resonance Energy Transfer-Based Sensor Camui Provides New Insight Into Mechanisms of Calcium/Calmodulin-Dependent Protein Kinase II Activation in Intact Cardiomyocytes [J].
Erickson, Jeffrey R. ;
Patel, Ruchi ;
Ferguson, Amanda ;
Bossuyt, Julie ;
Bers, Donald M. .
CIRCULATION RESEARCH, 2011, 109 (07) :729-U50
[9]   Lead-induced ER calcium release and inhibitory effects of methionine choline in cultured rat hippocampal neurons [J].
Fan, Guangqin ;
Zhou, Fankun ;
Feng, Chang ;
Wu, Fengyun ;
Ye, Weiwei ;
Wang, Chunhong ;
Lin, Fen ;
Yan, Ji ;
Li, Yanshu ;
Chen, Ying ;
Bi, Yongyi .
TOXICOLOGY IN VITRO, 2013, 27 (01) :387-395
[10]   The study of ISO induced heart failure rat model [J].
Feng, Weiwei ;
Li, Weimin .
EXPERIMENTAL AND MOLECULAR PATHOLOGY, 2010, 88 (02) :299-304
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