Cis-acting sequences required for light-responsive expression of the psbDII gene in Synechococcus sp. strain PCC 7942

被引：13

作者：

Anandan, S ^{[1
]}

Golden, SS ^{[1
]}

机构：

[1] TEXAS A&M UNIV,DEPT BIOL,COLLEGE STN,TX 77843

来源：

JOURNAL OF BACTERIOLOGY | 1997年 / 179卷 / 21期

关键词：

D O I：

10.1128/jb.179.21.6865-6870.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We analyzed the sequences required for promoter activity and high-light responsiveness of the psbDII gene in the cyanobacterium Synechococcus sp. strain PCC 7942 by using transcriptional fusions to a lacZ reporter gene. The basal promoter drives high constitutive expression, although no canonical -35 element is evident. The smallest fragment that showed clear light-responsive expression extends from -38 to +160, which includes 52 bp of the psbDII open reading frame. Sequences downstream from the promoter, within the untranslated leader region from +11 to +24, were required for high-light induction.

引用

页码：6865 / 6870

页数：6

共 25 条

[1]

ANANDAN S, UNPUB

[2]

Ausubel FM, 1995, SHORT PROTOCOLS MOL

[3]

BRYANT DA, 1987, CAN B FISH AQUAT SCI, V214, P423

[4] EXPRESSION OF THE PSBDII GENE IN SYNECHOCOCCUS SP STRAIN-PCC-7942 REQUIRES SEQUENCES DOWNSTREAM OF THE TRANSCRIPTION START SITE [J].