Photodynamic inactivation of enveloped virus in protein plasma preparations by solid-phase fullerene-based photosensitizer

被引：12

作者：

Belousova, I. M. ^{[1
]}

Kislyakov, I. M. ^{[1
]}

Muraviova, T. D. ^{[1
]}

Starodubtsev, A. M. ^{[1
]}

Kris'ko, T. K. ^{[1
]}

Selivanov, E. A. ^{[2
]}

Sivakova, N. P. ^{[2
]}

Golovanova, I. S. ^{[2
]}

Volkova, S. D. ^{[2
]}

Shtro, A. A. ^{[3
]}

Zarubaev, V. V. ^{[3
]}

机构：

[1] SI Vavilov State Opt Inst, St Petersburg, Russia

[2] Inst Hematol & Transfusiol, St Petersburg, Russia

[3] Influenza Res Inst, St Petersburg, Russia

来源：

PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY | 2014年 / 11卷 / 02期

关键词：

Blood products; Enveloped virus; Photoinactivation; Fullerene C60; SOLVENT DETERGENT; SINGLET OXYGEN; C-60; TRANSFUSION; RISK;

D O I：

10.1016/j.pdpdt.2014.02.009

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: The problem of transfution-transmitted infections still remains serious and actual for health care despite the detailed testing of donors. Human immunodeficiency virus, hepatitis B and C viruses and human cytomegalovirus are among the most dangerous pathogens that can be transmitted with blood. Previously, a composition consisting of fullerene layer applied on silica gel particles was shown to inactivate influenza virus up to complete loss of infectivity. Methods: In the present study the unit has been developed with source of irradiation whose spectrum is appropriate for solid-phase fullerene. The ability of the unit to inactivate the enveloped influenza virus in protein fraction of donor blood has been studied. Results: It was shown that at optimized conditions complete inactivation of enveloped virus of extremely high initial titer (7.0-9.5 log(10)EID(50)/0.2 mL) in the solution of albumin was achieved after as short time as 30 min of irradiation. This process did not affect the oxidative metabolism of neutrophils and membranes of erythrocytes evaluated by NBT reduction test and morphological analysis of erythrocytes, respectively. Conclusion: The data obtained suggests that the method described can be recommended for further development and optimization of the procedure of inactivation of viruses in the preparations of the plasma of donor blood. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：165 / 170

页数：6

共 21 条

[1] PHOTOPHYSICAL PROPERTIES OF C60 [J].