Expression of ADAM-17, TIMP-3 and fractalkine in the human adult brain endothelial cell line, hCMEC/D3, following pro-inflammatory cytokine treatment

被引：14

作者：

Hurst, Louise A. ^{[1
]}

Bunning, Rowena A. D. ^{[1
]}

Couraud, Pierre-Olivier ^{[2
,3
]}

Romero, Ignacio A. ^{[4
]}

Weksler, Babette B. ^{[5
]}

Sharrack, Basil ^{[6
]}

Woodroofe, M. Nicola ^{[1
]}

机构：

[1] Sheffield Hallam Univ, Biomed Res Ctr, Sheffield S1 1WB, S Yorkshire, England

[2] Univ Paris 05, Inst Cochin, CNRS, UMR 8104, Paris, France

[3] INSERM, U567, Paris, France

[4] Open Univ, Dept Life Sci, Milton Keynes MK7 6AA, Bucks, England

[5] Cornell Univ, Weill Med Coll, Div Hematol Med Oncol, New York, NY 10065 USA

[6] Royal Hallamshire Hosp, Dept Neurol, Sheffield S10 2JF, S Yorkshire, England

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2009年 / 210卷 / 1-2期

关键词：

ADAM-17; Multiple sclerosis; Fractalkine; Endothelial cells; ALPHA-CONVERTING-ENZYME; NECROSIS-FACTOR-ALPHA; EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; INTERCELLULAR-ADHESION MOLECULE-1; MEMBRANE-BOUND CHEMOKINE; TNF-ALPHA; CX(3)CR1 EXPRESSION; CX3CL1; FRACTALKINE; METALLOPROTEINASE; DISINTEGRIN;

D O I：

10.1016/j.jneuroim.2009.02.008

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

ADAM-17 expression is localised to endothelial cells in the human central nervous system (CNS) and is increased in multiple sclerosis (MS) white matter, suggesting a role in MS pathogenesis. Expression of ADAM-17, TIMP-3, and fractalkine were investigated in a human brain endothelial cell line (hCMEC/D3) after pro-inflammatory cytokine treatment. Tumour necrosis factor (TNF) significantly increased fractalkine mRNA (> 100 fold) and protein expression, which was associated with increased shedding of fractalkine from the cell. Fractalkine shedding may regulate immune cell trafficking into the CNS, however, this does not appear to be directly controlled by ADAM-17 activity. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：108 / 112

页数：5

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