Sortase-Mediated Ligation: A Gift from Gram-Positive Bacteria to Protein Engineering

被引：167

作者：

Tsukiji, Shinya ^{[1
]}

Nagamune, Teruyuki ^{[1
,2
,3
]}

机构：

[1] Univ Tokyo, Dept Chem & Biotechnol, Sch English, Bunkyo Ku, Tokyo 1138656, Japan

[2] Univ Tokyo, Dept Bioengn, Sch Engn, Bunkyo Ku, Tokyo 1138656, Japan

[3] Univ Tokyo, Ctr NanoBio Integrat, Bunkyo Ku, Tokyo 1138656, Japan

来源：

CHEMBIOCHEM | 2009年 / 10卷 / 05期

关键词：

bioconjugation; bioorganic chemistry; ligases; protein engineering; protein ligation; STAPHYLOCOCCUS-AUREUS SORTASE; RESONANCE ENERGY-TRANSFER; SURFACE-PROTEINS; CELL-WALL; SPLIT INTEIN; SUBSTRATE-SPECIFICITY; MANIPULATING PROTEINS; RECOMBINANT PROTEIN; CHEMICAL LIGATION; PEPTIDE LIGATION;

D O I：

10.1002/cbic.200800724

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：787 / 798

页数：12

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