Enhanced nonenzymatic sensing of hydrogen peroxide released from living cells based on Fe3O4/self-reduced graphene nanocomposites

被引:40
作者
Fang, Heting [1 ]
Pan, Yuliang [1 ]
Shan, Wenqian [1 ]
Guo, Manli [1 ,2 ]
Nie, Zhou [1 ]
Huang, Yan [1 ]
Yao, Shouzhuo [1 ]
机构
[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China
[2] S China Normal Univ, Sch Chem & Environm, Guangzhou 510006, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
OXIDATIVE STRESS; ENZYME-FREE; MAGNETIC NANOPARTICLES; AMPEROMETRIC SENSOR; EXTRACELLULAR H2O2; OXIDE; CYTOTOXICITY; GENERATION; ELECTRODE; MECHANISM;
D O I
10.1039/c4ay00549j
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A novel, sensitive, nonenzymatic amperometric sensor for the reliable detection of extracellular H2O2 released from living cells was fabricated based on Fe3O4/reduced graphene oxide (Fe3O4/rGO) nanocomposites, which were prepared using a simple and cost-effective one-pot approach. Electrochemical performances of the Fe3O4/rGO nanocomposites modified glassy carbon electrode (GCE) were studied. The results demonstrated that this H2O2 sensor exhibited excellent electrocatalytic performance towards the reduction of H2O2 at a potential of -0.3 V in the wide linear concentration range from 0.001 to 20 mM with a high sensitivity of 387.6 mu A mM(-1) cm(-2) and a detection limit as low as 0.17 mu M (S/N = 3), which was lower than certain enzymes and noble metal nanomaterials-based biosensors. Moreover, good anti-interference property, reproducibility, and long-term stability of the enzymeless sensor were achieved. Because of these remarkable analytical advantages, a novel, effective approach for the detection of extracellular H2O2 released from HeLa cells stimulated by CdTe quantum dots (QDs) was established by the constructed sensor. Since H2O2 is a byproduct of several oxidative biological reactions, this work could be applied to study the downstream biological effects of various stimuli in pathophysiology, and may expand the application of Fe3O4-based nanomaterials in the field of electrochemical sensing and bioanalysis.
引用
收藏
页码:6073 / 6081
页数:9
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