Single-column purification of recombinant human neurotrophin-3 (hNT3) by using the intein mediated self-cleaving system

被引：3
作者：
Li, ZY [1 ]
Fan, JH [1 ]
Yuan, JM [1 ]
机构：
[1] Shanxi Univ, Inst Biotechnol, Shanxi 030006, Taiyuang, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 20期
关键词：
affinity purification; bioactivity; human neurotrophin-3; intein;
D O I：
10.1023/A:1020692530678
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The gene of human neurotrophin-3 (hNT3), a 380 bp fragment corresponding to a 14 kDa protein, was amplified by PCR with genome DNA of human whole blood as the template and cloned into a vector pTXB1. The recombinant including the hNT3 gene was expressed in E. coli and the target product was in the form of inclusion bodies. After denaturation and renaturation, the hNT3 was purified on an affinity column by a self-cleaving intein. The bioactivity assay showed that the purified hNT3 induced profuse neurite outgrowth from the dorsal root ganglia (DRG) explants.
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收藏
页码：1723 / 1727
页数：5
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