Genetic Detection of Extended-Spectrum β-Lactamase-Containing Escherichia coli Isolates and Vancomycin-Resistant Enterococci in Fecal Samples of Healthy Children

被引：37

作者：

Guimaraes, Bruno ^{[1
,2
,3
,4
]}

Barreto, Angela ^{[1
,2
,3
,4
]}

Radhouani, Hajer ^{[1
,2
,3
,4
]}

Figueiredo, Nicholas ^{[1
,2
,3
,4
]}

Gaspar, Eurico ^{[5
]}

Rodrigues, Jorge ^{[1
,2
]}

Torres, Carmen ^{[6
]}

Igrejas, Gilberto ^{[3
,4
]}

Poeta, Patricia ^{[1
,2
]}

机构：

[1] Univ Tras os Montes & Alto Douro, Dept Vet Sci, P-5000911 Vila Real, Portugal

[2] Ctr Studies Anim & Vet Sci, Vila Real, Portugal

[3] Univ Tras os Montes & Alto Douro, Dept Genet & Biotechnol, P-5000911 Vila Real, Portugal

[4] Univ Tras os Montes & Alto Douro, Ctr Genet & Biotechnol, Inst Biotechnol & Bioengn, P-5000911 Vila Real, Portugal

[5] Hosp Tras os Montes & Alto Douro, Serv Pediat, Vila Real, Portugal

[6] Univ La Rioja, Biochem & Mol Biol Area, Logrono, Spain

来源：

MICROBIAL DRUG RESISTANCE | 2009年 / 15卷 / 03期

关键词：

CTX-M; HUMANS; ANIMALS; ENTEROBACTERIACEAE; DIVERSITY; STRAINS; FAECIUM; PREVALENCE; MECHANISMS; PORTUGAL;

D O I：

10.1089/mdr.2009.0910

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

One hundred twelve fecal samples of healthy children were recovered in Portugal during October 2007 and February 2008 and were tested for extended-spectrum beta-lactamases (ESBLs) containing Escherichia coli isolates and vancomycin-resistant enterococci (VRE). Three of the 112 fecal samples (2.7%) harbored ESBL-positive E. coli isolates and the bla(CTX-M-1), bla(TEM-52), and bla(SHV-12) genes were identified in these isolates. The bla(TEM-52)-containing isolate showed a phenotype of multiresistance that included fluoroquinolones, tetracycline, trimethoprim-sulfamethoxazole, and chloramphenicol; sul1, sul3, and cmlA genes were detected in this isolate, in addition to two amino acid changes in GyrA (Ser83Leu + Asp87Asn) and one in ParC protein (Ser80Ile). The ESBL isolates corresponded to phylogroup A (one isolate), B1 (one isolate), and D (one isolate). vanA-containing Escherichia faecium isolates were detected in 13 of the 112 fecal samples (11.6%), and vanC-1 isolates were found in 2 samples. A diversity of resistance genes [(tet(M), tet(L), erm(B), aph(3 ')-IIIa, ant(6)-Ia, catA, and vat(E)] were found in VRE isolates. These results show that the intestinal tract of healthy children constitutes a reservoir of ESBL-containing E. coli and VRE isolates.

引用

页码：211 / 216

页数：6

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