A selective fluorescent sensor for detecting lead in living cells

被引：335
作者：
He, Qiwen [1 ]
Miller, Evan W. [1 ]
Wong, Audrey P. [1 ]
Chang, Christopher J. [1 ]
机构：
[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 29期
关键词：
PB2+; ION; CHEMOSENSOR; BIOSENSOR; MECHANISM; DNAZYME; PB(II);
D O I：
10.1021/ja063029x
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We present the synthesis, properties, and biological applications of Leadfluor-1 (LF1), a new water-soluble, turn-on fluorescent sensor that is capable of selectively imaging Pb2+ in aqueous solution and in living cells. LF1 combines a fluorescein chromophore and a pseudocrown receptor to provide good selectivity for Pb2+ over a range of biologically and environmentally relevant metal ions in aqueous solution, with sensitivity to parts per billion EPA limits for allowable lead in drinking water. In addition to these attributes, imaging experiments further show that LF1 is the first small-molecule reagent that can be delivered into living cells and report changes in intracellular Pb2+ levels. Copyright © 2006 American Chemical Society.
引用
收藏
页码：9316 / 9317
页数：2
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