Gene-specific silencing induced by parallel complementary RNA in Pseudomonas aeruginosa

被引：5
|
作者：
Liu, Yi Shuang [1 ,2 ]
Zhang, Yue Qin [1 ,2 ]
Yang, Li Xia [1 ,2 ]
Yao, Tian Jue [1 ,2 ]
Xiao, Chun Ling [1 ,2 ]
机构：
[1] Chinese Acad Med Sci, Inst Med Biotechnol, Beijing 100050, Peoples R China
[2] Peking Union Med Coll, Beijing 100050, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2009年 / 31卷 / 10期
关键词：
Efflux pump; Gene-specific silencing; mexA; Parallel complementary RNA; Pseudomonas aeruginosa; MEXA-MEXB-OPRM; EXPRESSION; MACHINERY; SYSTEM;
D O I：
10.1007/s10529-009-0046-x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
To investigate whether parallel complementary RNA (pRNA) could induce gene-specific silencing in Pseudomonas aeruginosa, pRNA of the mexA gene was expressed in it. Compared to the control strains, the strain expressing pRNA of mexA showed a 50% decrease in minimum inhibitory concentrations (MICs) of several antimicrobial agents and a twofold increase in the initial accumulation rate of ethidium bromide, all of which are substrates of the MexAB-OprM efflux pump. These results suggest that gene-specific silencing was induced by pRNA. This is the first time that such a route for gene silencing has been reported in a bacterium other than Escherichia coli. Gene-specific silencing induced by pRNA may be useful as a novel biotechnology tool for gene regulation in prokaryotes.
引用
收藏
页码：1571 / 1575
页数：5
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