Goat RSPO1 over-expression rescues sex-reversal in Rspo1-knockout XX mice but does not perturb testis differentiation in XY or sex-reversed XX mice

被引：14

作者：

Buscara, Laurine ^{[1
]}

Montazer-Torbati, Fatemeh ^{[2
]}

Chadi, Sead ^{[1
]}

Auguste, Aurelie ^{[2
]}

Laubier, Johann ^{[1
]}

Chassot, Anne-Amandine ^{[3
,4
]}

Renault, Lauriane ^{[2
]}

Passet, Bruno ^{[1
]}

Costa, Jose ^{[1
]}

Pannetier, Maelle ^{[2
]}

Vilotte, Marthe ^{[1
]}

Chaboissier, Marie-Christine ^{[3
,4
]}

Vilotte, Jean-Luc ^{[1
]}

Pailhoux, Eric ^{[2
]}

Le Provost, Fabienne ^{[1
]}

机构：

[1] INRA, UR339, Lab Genet Bioquim & Cytigenet, F-78350 Jouy En Josas, France

[2] INRA, UMR1198, F-78350 Jouy En Josas, France

[3] Univ Nice Sophia Antipolis, U636, INSERM, F-06108 Nice, France

[4] Univ Nice Sophia Antipolis, Lab Genet Dev Normal & Pathol, F-06108 Nice, France

来源：

TRANSGENIC RESEARCH | 2009年 / 18卷 / 04期

关键词：

Sex determination; R-Spondin1; Transgenesis; BAC; BETA-CATENIN; TRANSGENIC MICE; OVARIAN DEVELOPMENT; R-SPONDIN1; WNT4; SRY; TRANSCRIPTION; CHROMOSOME; GONAD; LRP6;

D O I：

10.1007/s11248-009-9247-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

RSPO1 is a newly discovered gene involved in sex differentiation. Two goat BAC clones encompassing the RSPO1 gene (gRSPO1) were injected into mouse oocytes and several transgenic lines derived. Both clones induced gRSPO1 over-expression in various tissues, including male and female gonads, with no obvious phenotype and normal sex-ratios. Introgression of the gRSPO1 transgene into a mouse RSPO1 knockout genotype resulted in the rescue of the fertility and the disappearance of the masculinized gonadic features of the females, demonstrating the functionality of the goat protein in a mouse context. On the contrary, over-expression of gRSPO1 within a mSRY or a gSRY-XX genotypes did not interfere with the SRY-induced male phenotype.

引用

页码：649 / 654

页数：6

共 27 条

[11] R-spondin1 and FOXL2 act into two distinct cellular types during goat ovarian differentiation [J].

Kocer, Ayhan ;

Pinheiro, Iris ;

Pannetier, Maelle ;

Renault, Lauriane ;

Parma, Pietro ;

Radi, Orietta ;

Kim, Kyung-Ah ;

Camerino, Giovanna ;

Pailhoux, Eric .

BMC DEVELOPMENTAL BIOLOGY, 2008, 8

[12] MALE DEVELOPMENT OF CHROMOSOMALLY FEMALE MICE TRANSGENIC FOR SRY [J].

KOOPMAN, P ;

GUBBAY, J ;

VIVIAN, N ;

GOODFELLOW, P ;

LOVELLBADGE, R .

NATURE, 1991, 351 (6322) :117-121

[13] Stabilization of β-catenin in XY gonads causes male-to-female sex-reversal [J].

Maatouk, Danielle M. ;

DiNapoli, Leo ;

Alvers, Ashley ;

Parker, Keith L. ;

Taketo, Makoto M. ;

Capel, Blanche .

HUMAN MOLECULAR GENETICS, 2008, 17 (19) :2949-2955

[14] Dax-1 (dosage-sensitive sex reversal-adrenal hypoplasia congenita critical region on the X chromosome, gene 1) gene transcription is regulated by Wnt4 in the female developing gonad [J].

Mizusaki, H ;

Kawabe, K ;

Mukai, T ;

Ariyoshi, E ;

Kasahara, M ;

Yoshioka, H ;

Swain, A ;

Morohashi, K .

MOLECULAR ENDOCRINOLOGY, 2003, 17 (04) :507-519

[15] Dynamic expression of R-spondin family genes in mouse development [J].

Nam, Ju-Suk ;

Turcotte, Taryn J. ;

Yoon, Jeong Kyo .

GENE EXPRESSION PATTERNS, 2007, 7 (03) :306-312

[16] Loss of Wnt4 and Foxl2 leads to female-to-male sex reversal extending to germ cells [J].

Ottolenghi, Chris ;

Pelosi, Emanuele ;

Tran, Joseph ;

Colombino, Maria ;

Douglass, Eric ;

Nedorezov, Timur ;

Cao, Antonio ;

Forabosco, Antonino ;

Schlessinger, David .

HUMAN MOLECULAR GENETICS, 2007, 16 (23) :2795-2804

[17] A 11.7-kb deletion triggers intersexuality and polledness in goats [J].

Pailhoux, E ;

Vigier, B ;

Chaffaux, S ;

Servel, N ;

Taourit, S ;

Furet, JP ;

Fellous, M ;

Grosclaude, F ;

Cribiu, EP ;

Cotinot, C ;

Vaiman, D .

NATURE GENETICS, 2001, 29 (04) :453-458

[18] Goat SRY induces testis development in XX transgenic mice [J].

Pannetier, Maelle ;

Tilly, Gaelle ;

Kocer, Ayhan ;

Hudrisier, Marthe ;

Renault, Lauriane ;

Chesnais, Nathalie ;

Costa, Jose ;

Le Provost, Fabienne ;

Vaiman, Daniel ;

Vilotte, Jean-Luc ;

Pailhoux, Eric .

FEBS LETTERS, 2006, 580 (15) :3715-3720

[19] R-spondin1 is essential in sex determination, skin differentiation and malignancy [J].

Parma, Pietro ;

Radi, Orietta ;

Vidal, Valerie ;

Chaboissier, Marie Christine ;

Dellambra, Elena ;

Valentini, Stella ;

Guerra, Liliana ;

Schedl, Andreas ;

Camerino, Giovanna .

NATURE GENETICS, 2006, 38 (11) :1304-1309

[20] A METHOD FOR THE GENERATION OF YAC TRANSGENIC MICE BY PRONUCLEAR MICROINJECTION [J].

SCHEDL, A ;

LARIN, Z ;

MONTOLIU, L ;

THIES, E ;

KELSEY, G ;

LEHRACH, H ;

SCHUTZ, G .

NUCLEIC ACIDS RESEARCH, 1993, 21 (20) :4783-4787

← 1 2 3 →