Molecular characterization of Salmonella Typhimurium isolated in Brazil by CRISPR-MVLST

被引：23

作者：

Almeida, Fernanda ^{[1
]}

Medeiros, Marta Ines Cazentini ^{[2
]}

Rodrigues, Dalia dos Prazeres ^{[3
]}

Allard, Marc W. ^{[4
]}

Falcao, Juliana Pfrimer ^{[1
]}

机构：

[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP, Brazil

[2] Adolfo Lutz Inst, Ctr Lab Reg Ribeirao Preto, Rua Minas 877, Ribeirao Preto, SP, Brazil

[3] FIOCRUZ Fundacao Inst Oswaldo Cruz, Lab Enterobacterias, Ave Brasil,4365,Pavilhao Rocha Lima,3 Andar, BR-21040360 Rio De Janeiro, RJ, Brazil

[4] US FDA, Off Regulatory Sci, Ctr Food Safety & Appl Nutr, College Pk, MD USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2017年 / 133卷

基金：

巴西圣保罗研究基金会;

关键词：

CRISPR-MVLST; PFGE; Salmonella Typhimurium; SUBSP ENTERICA SEROVAR; VIRULENCE-ASSOCIATED GENES; FIELD GEL-ELECTROPHORESIS; SAO-PAULO STATE; ANTIMICROBIAL RESISTANCE; ENTERITIDIS STRAINS; HUMANS; FOOD; DIVERSITY; MARKERS;

D O I：

10.1016/j.mimet.2016.12.020

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

CRISPR-multi-locus virulence sequence typing (CRISPR-MVLST) was performed to type 92 S. Typhimurium strains isolated from humans and food sources between 1983 and 2013 in Brazil and assess the suitability of this methodology comparing it with PFGE already used for subtyping the same strains. Among the 92 S. Typhimurium strains studied, we identified 25 CRISPR1 alleles, 27 CRISPR2 alleles, 2fimH alleles and 3 sseL alleles showing that the genetic variability is much higher in the CRISPRs loci than in the virulence genes. The CRISPR-MVLST analysis provided similar results to the PFGE previously published used to type the same set of strains, demonstrating that CRISPR-MVLST is a very efficient approach for subtyping S. Typhimurium serovar and can be used to complement and validate results obtained by the PFGE methodology. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：55 / 61

页数：7

共 34 条

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