Molecular characterization of Salmonella Typhimurium isolated in Brazil by CRISPR-MVLST

被引：23
作者：
Almeida, Fernanda [1 ]
Medeiros, Marta Ines Cazentini [2 ]
Rodrigues, Dalia dos Prazeres [3 ]
Allard, Marc W. [4 ]
Falcao, Juliana Pfrimer [1 ]
机构：
[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP, Brazil
[2] Adolfo Lutz Inst, Ctr Lab Reg Ribeirao Preto, Rua Minas 877, Ribeirao Preto, SP, Brazil
[3] FIOCRUZ Fundacao Inst Oswaldo Cruz, Lab Enterobacterias, Ave Brasil,4365,Pavilhao Rocha Lima,3 Andar, BR-21040360 Rio De Janeiro, RJ, Brazil
[4] US FDA, Off Regulatory Sci, Ctr Food Safety & Appl Nutr, College Pk, MD USA
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2017年 / 133卷
基金：
巴西圣保罗研究基金会;
关键词：
CRISPR-MVLST; PFGE; Salmonella Typhimurium; SUBSP ENTERICA SEROVAR; VIRULENCE-ASSOCIATED GENES; FIELD GEL-ELECTROPHORESIS; SAO-PAULO STATE; ANTIMICROBIAL RESISTANCE; ENTERITIDIS STRAINS; HUMANS; FOOD; DIVERSITY; MARKERS;
D O I：
10.1016/j.mimet.2016.12.020
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
CRISPR-multi-locus virulence sequence typing (CRISPR-MVLST) was performed to type 92 S. Typhimurium strains isolated from humans and food sources between 1983 and 2013 in Brazil and assess the suitability of this methodology comparing it with PFGE already used for subtyping the same strains. Among the 92 S. Typhimurium strains studied, we identified 25 CRISPR1 alleles, 27 CRISPR2 alleles, 2fimH alleles and 3 sseL alleles showing that the genetic variability is much higher in the CRISPRs loci than in the virulence genes. The CRISPR-MVLST analysis provided similar results to the PFGE previously published used to type the same set of strains, demonstrating that CRISPR-MVLST is a very efficient approach for subtyping S. Typhimurium serovar and can be used to complement and validate results obtained by the PFGE methodology. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：55 / 61
页数：7
相关论文
共 34 条
  • [1] Virulence-associated genes, antimicrobial resistance and molecular typing of Salmonella Typhimurium strains isolated from swine from 2000 to 2012 in Brazil
    Almeida, F.
    Medeiros, M. I. C.
    Kich, J. D.
    Falcao, J. P.
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2016, 120 (06) : 1677 - 1690
  • [2] Genotypic diversity, pathogenic potential and the resistance profile of Salmonella Typhimurium strains isolated from humans and food from 1983 to 2013 in Brazil
    Almeida, Fernanda
    Cazentini Medeiros, Marta Ines
    Rodrigues, Dalia dos Prazeres
    Falcao, Juliana Pfrimer
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2015, 64 : 1395 - 1407
  • [3] Molecular epidemiology and virulence markers of Salmonella Infantis isolated over 25 years in Sao Paulo State, Brazil
    Almeida, Fernanda
    Pitondo-Silva, Andre
    Oliveira, Maria Aparecida
    Falcao, Juliana Pfrimer
    [J]. INFECTION GENETICS AND EVOLUTION, 2013, 19 : 145 - 151
  • [4] Phenotypic and genetic characterization of Salmonella enterica subsp enterica serovar Typhimurium isolated from pigs in Rio Grande!do Sul, Brazil
    Bessa, Marjo Cado
    Michael, Geovana Brenner
    Canu, Nunzia
    Canal, Claudio Wageck
    Cardoso, Marisa
    Rabsch, Wolfgang
    Rubino, Salvatore
    [J]. RESEARCH IN VETERINARY SCIENCE, 2007, 83 (03) : 302 - 310
  • [5] Comparison of four molecular methods to type Salmonella Enteritidis strains
    Campioni, Fabio
    Pitondo-Silva, Andre
    Bergamini, Alzira M. M.
    Falcao, Juliana P.
    [J]. APMIS, 2015, 123 (05) : 422 - 426
  • [6] Genotyping of Yersinia enterocolitica biotype 1A strains from clinical and nonclinical origins by pulsed-field gel electrophoresis
    Campioni, Fabio
    Falcao, Juliana P.
    [J]. CANADIAN JOURNAL OF MICROBIOLOGY, 2014, 60 (06) : 419 - 424
  • [7] MLVA typing reveals higher genetic homogeneity among S. Enteritidis strains isolated from food, humans and chickens in Brazil in comparison to the North American Strains
    Campioni, Fabio
    Davis, Margaret
    Medeiros, Marta Ines C.
    Falcao, Juliana P.
    Shah, Devendra H.
    [J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2013, 162 (02) : 174 - 181
  • [8] Genetic diversity, virulence genes and antimicrobial resistance of Salmonella Enteritidis isolated from food and humans over a 24-year period in Brazil
    Campioni, Fabio
    Moratto Bergamini, Alzira Maria
    Falcao, Juliana P.
    [J]. FOOD MICROBIOLOGY, 2012, 32 (02) : 254 - 264
  • [9] Comparative Analysis of Subtyping Methods against a Whole-Genome-Sequencing Standard for Salmonella enterica Serotype Enteritidis
    Deng, Xiangyu
    Shariat, Nikki
    Driebe, Elizabeth M.
    Roe, Chandler C.
    Tolar, Beth
    Trees, Eija
    Keim, Paul
    Zhang, Wei
    Dudley, Edward G.
    Fields, Patricia I.
    Engelthaler, David M.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2015, 53 (01) : 212 - 218
  • [10] Dimarzio M., 2013, ANTIMICROB AGENTS CH
1234