Alterations in Intestinal Microbiota of Children With Celiac Disease at the Time of Diagnosis and on a Gluten-free Diet

被引：50

作者：

Zafeiropoulou, Konstantina ^{[1
]}

Nichols, Ben ^{[1
]}

Mackinder, Mary ^{[1
]}

Biskou, Olga ^{[1
]}

Rizou, Eleni ^{[1
]}

Karanikolou, Antonia ^{[1
]}

Clark, Clare ^{[1
]}

Buchanan, Elaine ^{[2
]}

Cardigan, Tracey ^{[2
]}

Duncan, Hazel ^{[2
]}

Wands, David ^{[2
]}

Russell, Julie ^{[3
]}

Hansen, Richard ^{[2
]}

Russell, Richard K. ^{[2
]}

McGrogan, Paraic ^{[2
]}

Edwards, Christine A. ^{[1
]}

Ijaz, Umer Z. ^{[3
]}

Gerasimidis, Konstantinos ^{[1
]}

机构：

[1] Univ Glasgow, Glasgow Royal Infirm, Coll Med Vet & Life Sci, Sch Med Dent & Nursing,Human Nutr, New Lister Bldg, Glasgow G31 2ER, Lanark, Scotland

[2] Royal Hosp Children, Dept Paediat Gastroenterol, Glasgow, Lanark, Scotland

[3] Univ Glasgow, Sch Engn, Civil Engn, Glasgow, Lanark, Scotland

来源：

GASTROENTEROLOGY | 2020年 / 159卷 / 06期

基金：

英国生物技术与生命科学研究理事会;

关键词：

OTU; Pediatric; Microbiome; Short-chain Fatty Acids;

D O I：

10.1053/j.gastro.2020.08.007

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

BACKGROUND AND AIMS: It is not clear whether alterations in the intestinal microbiota of children with celiac disease (CD) cause the disease or are a result of disease and/or its treatment with a gluten-free diet (GFD). METHODS: We obtained 167 fecal samples from 141 children (20 with new onset CD, 45 treated with a GFD, 57 healthy children, and 19 unaffected siblings of children with CD) in Glasgow, Scotland. Samples were analyzed by 16S ribosomal RNA sequencing, and diet-related metabolites were measured by gas chromatography. We obtained fecal samples from 13 children with new-onset CD after 6 and 12 months on a GFD. Relationships between microbiota with diet composition, gastrointestinal function, and biomarkers of GFD compliance were explored. RESULTS: Microbiota a diversity did not differ among groups. Microbial dysbiosis was not observed in children with new-onset CD. In contrast, 2.8% (Bray-Curtis dissimilarity index, P = .025) and 2.5% (UniFrac distances, P = .027) of the variation in microbiota composition could be explained by the GFD. Between 3% and 5% of all taxa differed among all group comparisons. Eleven distinctive operational taxonomic units composed a microbe signature specific to CD with high diagnostic probability. Most operational taxonomic units that differed between patients on a GFD with new-onset CD vs healthy children were associated with nutrient and food group intake (from 75% to 94%) and with biomarkers of gluten ingestion. Fecal levels of butyrate and ammonia decreased during the GFD. CONCLUSIONS: Although several alterations in the intestinal microbiota of children with established CD appear to be effects of a GFD, specific bacteria were found to be distinct biomarkers of CD. Studies are needed to determine whether these bacteria contribute to pathogenesis of CD.

引用

页码：2039 / 2051.e20

页数：33

共 48 条

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