Whole-comparative genomic hybridization in domestic sheep (Ovis aries) breeds

被引:9
作者
Davila-Rodriguez, M. I. [1 ]
Cortes-Gutierrez, E. I. [1 ]
Lopez-Fernandez, C. [2 ]
Pita, M. [2 ]
Mezzanotte, R. [3 ]
Gosalvez, J. [2 ]
机构
[1] Ctr Invest Biomed Noreste, Inst Mexicano Seguro Social, Dept Genet, Monterrey, Mexico
[2] Univ Autonoma Madrid, Dept Biol, Genet Unit, Madrid, Spain
[3] Cittadella Univ, Sez Biol & Genet, Dip Sci & Tecnol Biomed, Monserrato, Cagliari, Italy
关键词
ALKALI-LABILE SITES; REPETITIVE DNA-SEQUENCES; SATELLITE DNA; CONSTITUTIVE HETEROCHROMATIN; DBD-FISH; W-CGH; CHROMOSOMES; BREAKAGE; EVOLUTION; SPERM;
D O I
10.1159/000200084
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Whole-comparative genomic hybridization (W-CGH) allows identification of chromosomal polymorphisms related to highly repetitive DNA sequences localized in constitutive heterochromatin. Such polymorphisms are detected establishing competition between genomic DNAs in an in situ hybridization environment without subtraction of highly repetitive DNA sequences, when comparing two species from closely related taxa (same species, sub-species, or breeds) or somewhat related taxa. This experimental approach was applied to investigating differences in highly repetitive sequences of three sheep breeds (Castellana, Ojalada, and Assaf). To this end, W-CGH was carried out using mouflon (sheep ancestor) chromosomes as a common target to co-hybridize equimolar quantities of two genomic DNAs obtained from either Castellana, Ojalada or Assaf sheep breeds. The results showed that the amount of constitutive heterochromatin is greater in all pericentromeric heterochromatin regions of acrocentric chromosomes than in metacentric or sex chromosomes. Additionally, when W-CGH was performed using DNAs from the Iberian breeds Castellana and Ojalada, chromosomal pericentromeric regions revealed quantitatively and qualitatively a presence of DNA families similar to that obtained from any of the above-cited breeds. On the contrary, when the DNA used in W-CGH experiments was obtained from Assaf, as compared to either Castellana or Ojalada, two different pericentromeric DNA families of highly repetitive sequences could be detected. Lastly, sex chromosomes were shown to be homogeneous among all breeds and thus revealed no detectable constitutive heterochromatin. W-CGH results were confirmed using DNA breakage detection-FISH experiments (DBD-FISH) carried out on lymphocytes. As a whole, the results showed that two different repetitive DNA families are present in the pericentromeric heterochromatin of the sheep breeds studied here. Additionally, they suggest a differential presence of these distinct repetitive DNA families in Castellana and Ojalada breeds as compared to the Assaf breed. Finally, the results of W-CGH after using mouflon as the targeted chromosomes also show that the two DNA families are present in the ancestor. Copyright (C) 2009 S. Karger AG, Basel
引用
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页码:19 / 26
页数:8
相关论文
共 32 条
  • [1] Chromosome restriction enzyme digestion in domestic pig (Sus scrofa) constitutive heterochromatin arrangement
    Adega, F
    Chaves, R
    Guedes-Pinto, H
    [J]. GENES & GENETIC SYSTEMS, 2005, 80 (01) : 49 - 56
  • [2] CHARACTERIZATION OF THE HETEROCHROMATIN IN MOOSE (ALCES-ALCES) CHROMOSOMES
    BIANCHI, MS
    BIANCHI, NO
    GRIPENBERG, U
    WESSMAN, M
    HUUHTANEN, S
    [J]. GENETICA, 1990, 80 (01) : 1 - 7
  • [3] FUNDAMENTAL KARYOTYPE IN DOMESTIC AND WILD-SPECIES OF SHEEP - IDENTITY AND RANKING OF AUTOSOMAL ACROCENTRICS INVOLVED IN BIARMED FORMATIONS
    BUNCH, TD
    [J]. JOURNAL OF HEREDITY, 1978, 69 (02) : 77 - 80
  • [4] TRANSLOCATIONS OF ACROCENTRIC CHROMOSOMES AND THEIR IMPLICATIONS IN EVOLUTION OF SHEEP (OVIS)
    BUNCH, TD
    FOOTE, WC
    SPILLETT, JJ
    [J]. CYTOGENETICS AND CELL GENETICS, 1976, 17 (03): : 122 - 136
  • [5] LIVER CHROMATIN FRACTIONS IN MUS AND AKODON - THE CONCEPT OF CONSTITUTIVE HETEROCHROMATIN
    CATALA, A
    VIDALRIOJA, L
    BIANCHI, NO
    [J]. MOLECULAR AND CELLULAR BIOCHEMISTRY, 1981, 36 (03) : 135 - 141
  • [6] CHROMOSOME MORPHOLOGY DURING MEIOSIS OF NORMAL AND ROBERTSONIAN TRANSLOCATION-CARRYING RAMS (OVIS-ARIES)
    CHAPMAN, HM
    BRUERE, AN
    [J]. CANADIAN JOURNAL OF GENETICS AND CYTOLOGY, 1977, 19 (01): : 93 - 102
  • [7] The species and chromosomal distribution of the centromeric a-satellite I sequence from sheep in the tribe Caprini and other Bovidae
    Chaves, R
    Guedes-Pinto, H
    Heslop-Harrison, JS
    Schwarzacher, T
    [J]. CYTOGENETICS AND CELL GENETICS, 2000, 91 (1-4): : 62 - 66
  • [8] Alkali-labile sites in sperm cells from Sus and Ovis species
    Cortes-Gutierrez, Elva I.
    Davila-Rodriguez, Martha I.
    Lopez-Fernandez, Carmen
    Fernandez, Jose Luis
    Gosalvez, Jaime
    [J]. INTERNATIONAL JOURNAL OF ANDROLOGY, 2008, 31 (03): : 354 - 363
  • [9] DELAFUENTE LF, 2006, EUR ASS AN PROD EAAP, P17
  • [10] CHROMOSOME HOMOLOGY AND HETEROCHROMATIN IN GOAT, SHEEP AND OX STUDIED BY BANDING TECHNIQUES
    EVANS, HJ
    BUCKLAND, RA
    SUMNER, AT
    [J]. CHROMOSOMA, 1973, 42 (04) : 383 - 402