Cultivar identification and genetic relationship of mango (Mangifera indica) in Taiwan using 37 SSR markers

被引:21
作者
Tsai, Chi-Chu [1 ,2 ]
Chen, Y. U-Kuang H. [2 ]
Chen, Chih-Hsiung [3 ]
Weng, I. -Szu [1 ]
Tsai, Chi-Mou [2 ]
Lee, Sheue-Ru [1 ]
Lin, Yu-Shium [1 ]
Chiang, Yu-Chung [4 ]
机构
[1] Kaohsiung Dist Agr Res & Extens Stn, Pingtung 908, Taiwan
[2] Natl Pingtung Univ Sci & Technol, Pingtung 912, Taiwan
[3] Natl Museum Nat Sci, Dept Bot, Taichung 404, Taiwan
[4] Natl Sun Yat Sen Univ, Dept Biol Sci, Kaohsiung 804, Taiwan
关键词
Mango; Molecular marker; Genetic relationship; Cultivar identification; Embryonic characteristics; POLYMORPHIC MICROSATELLITE MARKERS; MULTILOCUS GENOTYPE DATA; POPULATION-STRUCTURE; GEOGRAPHIC DIFFERENTIATION; EMBRYO TYPE; L; INFERENCE; AMPLIFICATION; INTROGRESSION; LOCI;
D O I
10.1016/j.scienta.2013.09.037
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
The genetic relationship of twenty-two mango (Mangifera indica L.) cultivars/lines was examined based on 37 SSR markers. Numbers of alleles per locus of the 37 SSR markers ranged from 2 to 11, and a total of 182 alleles with an average of 4.86 alleles per locus. Banding patterns obtained from 37 SSR primers allowed for each cultivar/line to be distinguished from the others with the exception of vegetative clones. The result indicates that SSR analysis is an efficient method for cultivar identification. Similarity coefficients were calculated based on 182 amplified bands. The dendrogram and genetic structure was showed according to those SSR markers. As expected, the genetic relationship between derived cultivars/lines and their parental cultivars are closely related based on the dendrogram. However, the SSR markers tested cannot separate monoembryonic from polyembryonic seeds of the mango cultivars/lines based on both dendrogram and genetic structure in this study. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:196 / 201
页数:6
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