Cultivar identification and genetic relationship of mango (Mangifera indica) in Taiwan using 37 SSR markers

The genetic relationship of twenty-two mango (Mangifera indica L.) cultivars/lines was examined based on 37 SSR markers. Numbers of alleles per locus of the 37 SSR markers ranged from 2 to 11, and a total of 182 alleles with an average of 4.86 alleles per locus. Banding patterns obtained from 37 SSR primers allowed for each cultivar/line to be distinguished from the others with the exception of vegetative clones. The result indicates that SSR analysis is an efficient method for cultivar identification. Similarity coefficients were calculated based on 182 amplified bands. The dendrogram and genetic structure was showed according to those SSR markers. As expected, the genetic relationship between derived cultivars/lines and their parental cultivars are closely related based on the dendrogram. However, the SSR markers tested cannot separate monoembryonic from polyembryonic seeds of the mango cultivars/lines based on both dendrogram and genetic structure in this study. (C) 2013 Elsevier B.V. All rights reserved.