A route to low-cost nanoplasmonic biosensor integrated with optofluidic-portable platform

被引：25

作者：

Geng, Zhaoxin ^{[1
,2
]}

Kan, Qiang ^{[2
]}

Yuan, Jun ^{[2
]}

Chen, Hongda ^{[2
]}

机构：

[1] Minzu Univ China, Sch Informat Engn, Beijing 100081, Peoples R China

[2] Chinese Acad Sci, Inst Semicond, State Key Lab Integrated Optoelect, Beijing 100083, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2014年 / 195卷

基金：

中国国家自然科学基金;

关键词：

LSPR; Biosensor; Microfluidics; Nanoparticles; SURFACE-PLASMON RESONANCE;

D O I：

10.1016/j.snb.2014.01.110

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The optofluidic nanoplasmonic biosensors could offer label-free monitoring of biomolecular interactions in real-time with the low consumption of the sample, which stimulate researchers' tremendous interest. To meet the need of the laboratory and clinic testing, the protocols of fabricating nanoplasmonic biosensor and testing processes were detailedly described, which is simple and low-cost. The photolithography, electron beam evaporation, annealing and bonding were applied to fabricate the nanoplasmonic biosensor which integrated with microfluidics on an optofluidic-portable platform. The 9 kinds of samples with different reflective index and antigen/antibody system were utilized to characterize the developed nanoplasmonic biosensor. Meanwhile, the biomarker of the liver cancer also was tested in situ and real time by the optofluidic platform. The detected concentration of the liver cancer antigen and antibody reached 45.24 ng/ml and 25 ng/ml, respectively. This method could improve the diagnosing accuracy in the liver cancer early stage or other serious diseases. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：682 / 691

页数：10

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