Diagnostic Significance of Combined Detection of Epstein-Barr Virus Antibodies, VCA/IgA, EA/IgA, Rta/IgG and EBNA1/IgA for Nasopharyngeal Carcinoma

被引:25
作者
Cai, Yong-Lin [1 ]
Li, Jun [1 ]
Lu, Ai-Ying [1 ]
Zheng, Yu-Ming [1 ]
Zhong, Wei-Ming [2 ]
Wang, Wei [3 ]
Gao, Jian-Quan [2 ]
Zeng, Hong [1 ]
Cheng, Ji-Ru [1 ]
Tang, Min-Zhong [1 ,4 ]
机构
[1] Wuzhou Red Cross Hosp, Wuzhou Hlth Syst Key Lab Nasopharyngeal Carcinoma, Wuzhou, Guangxi Provinc, Peoples R China
[2] Wuzhou Red Cross Hosp, Dept Oncol, Wuzhou, Guangxi Provinc, Peoples R China
[3] Southern Med Univ, Nanfang Hosp, Res Ctr Clin Med, Guangzhou, Guangdong, Peoples R China
[4] Beijing Univ Technol, Coll Life Sci & Bioengn, Beijing, Peoples R China
关键词
Nasopharyngeal carcinoma; epstein-barr virus; antibody; tumor marker; serodiagnosis; VIRAL CAPSID ANTIGEN; RTA PROTEIN; EPITHELIAL-CELLS; SOUTHERN CHINA; IGA ANTIBODY; EXPRESSION; INFECTION; PATHOGENESIS; GENES; INDIVIDUALS;
D O I
10.7314/APJCP.2014.15.5.2001
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The objective of this study was to investigate the diagnostic significance of EBV antibody combined detection for nasopharyngeal carcinoma (NPC) in a high incidence region of southern China. Two hundred and eleven untreated NPC patients, 203 non-NPC ENT patients, and 210 healthy controls were recruited for the study. The titers of VCA/IgA and EA/IgA were assessed by immunoenzyme assay, and the levels of Rta/IgG and EBNA1/IgA were determined by enzyme-linked immunosorbent assay. The levels of VCA/IgA, EA/IgA, Rta/IgG and EBNA1/IgA demonstrated no association with gender or age (p>0.05). The receiver operating characteristic curve and the area under the curve were used to evaluate the diagnostic value. The sensitivity of VCA/IgA (98.1%) and the specificity of EA/IgA (98.5%) were the highest. When a logistic regression model was used to combine the results from multiple antibodies to increase the accuracy, the combination of VCA/IgA+Rta/IgG, whose area under the curve was 0.99, had the highest diagnostic efficiency, and its sensitivity, specificity and Youden index were 94.8%, 98.0% and 0.93 respectively. The data suggest that the combination of VCA/IgA+Rta/IgG may be most suitable for NPC serodiagnosis.
引用
收藏
页码:2001 / 2006
页数:6
相关论文
共 35 条
[1]   Epstein-Barr virus infection - life cycle, methods of diagnosis, associated diseases [J].
Bocian, Joanna ;
Januszkiewicz-Lewandowska, Danuta .
POSTEPY HIGIENY I MEDYCYNY DOSWIADCZALNEJ, 2011, 65 :286-298
[2]   Nasopharyngeal carcinoma [J].
Brennan, Bernadette .
ORPHANET JOURNAL OF RARE DISEASES, 2006, 1 (1)
[3]   Involvement of the Epstein-Barr virus in the nasopharyngeal carcinoma pathogenesis [J].
Burgos, JS .
MEDICAL ONCOLOGY, 2005, 22 (02) :113-121
[4]  
Cai Yong-lin, 2010, Nan Fang Yi Ke Da Xue Xue Bao, V30, P2746
[5]   EA-D p45-IgG as a Potential Biomarker for Nasopharyngeal Carcinoma Diagnosis [J].
Chen, Hao ;
Luo, Yao-Ling ;
Zhang, Lin ;
Tian, Li-Zhen ;
Feng, Zhi-Ting ;
Liu, Wan-Li .
ASIAN PACIFIC JOURNAL OF CANCER PREVENTION, 2013, 14 (12) :7433-7438
[6]   Assessing the risk of nasopharyngeal carcinoma on the basis of EBV antibody spectrum [J].
Cheng, WM ;
Chan, KH ;
Chen, HL ;
Luo, RX ;
Ng, SP ;
Luk, W ;
Zheng, BJ ;
Ji, MF ;
Liang, JS ;
Sham, JST ;
Wang, DK ;
Zong, YS ;
Ng, MH .
INTERNATIONAL JOURNAL OF CANCER, 2002, 97 (04) :489-492
[7]   Serologic markers of Epstein-Barr virus infection and nasopharyngeal carcinoma in Taiwanese men [J].
Chien, YC ;
Chen, JY ;
Liu, MY ;
Yang, HI ;
Hsu, MM ;
Chen, CJ ;
Yang, CS .
NEW ENGLAND JOURNAL OF MEDICINE, 2001, 345 (26) :1877-1882
[8]  
DENG H, 1995, CHINESE MED J-PEKING, V108, P300
[9]   Single-assay combination of Epstein-Barr virus (EBV) EBNA1-and viral capsid antigen-p18-derived synthetic peptides for measuring anti-EBV immunoglobulin G (IgG) and IgA antibody levels in sera from nasopharyngeal carcinoma patients: Options for field screening [J].
Fachiroh, J ;
Paramita, DK ;
Hariwiyanto, B ;
Harijadi, A ;
Dahlia, HL ;
Indrasari, SR ;
Kusumo, H ;
Zeng, YS ;
Schouten, T ;
Mubarika, S ;
Middeldorp, JM .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (04) :1459-1467
[10]   Expression of Epstein-Barr virus lytic gene BRLF1 in nasopharyngeal carcinoma: potential use in diagnosis [J].
Feng, P ;
Ren, EC ;
Liu, D ;
Chan, SH ;
Hu, H .
JOURNAL OF GENERAL VIROLOGY, 2000, 81 :2417-2423