Vitrification of precision-cut rat liver slices

被引：0
|
作者：
Ekins, S
机构：
来源：
CRYO-LETTERS | 1996年 / 17卷 / 01期
关键词：
rat liver slices; vitrification; 1,2-propanediol; cryocassette; 7-ethoxycoumarin; 7-hydroxycoumarin;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Previous studies have suggested vitrification as an appropriate method for long-term storage of tissues and this has recently been applied to preservation of human liver slices. I report the use of a cassette designed to store slices of liver or other tissues in combination with the previously described vitrification technique. Using 7-ethoxycoumarin and 7-hydroxycoumarin as markers of drug metabolising activity, I demonstrate the use of this apparatus and method over 1hr and 24hr storage with rat liver slices in liquid nitrogen.
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页码：7 / 14
页数：8
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