Leaf protein analysis during gibberellic and photoperiodic induction of flowering in Arabidopsis thaliana

Leaf proteins from Arabidopsis thaliana Landsberg erecta treated with gibberellin A(3) and different light regimes (continuous light, 16/8 h dark/light cycle and 8/16 h dark/light cycle) were analysed by two-dimensional gel electrophoresis. Qualitative differences between the controls and the treated plants were found, indicating that these treatments were responsible for the synthesis of specific proteins. A 35 kDa polypeptide is induced by gibberellin A(3) application and a 25 kDa polypeptide depends on the photoperiod.