Profile of new green fluorescent protein transgenic Jinhua pigs as an imaging source

被引：28

作者：

Kawarasaki, Tatsuo ^{[1
]}

Uchiyama, Kazuhiko ^{[2
]}

Hirao, Atsushi ^{[3
]}

Azuma, Sadahiro ^{[2
]}

Otake, Masayoshi ^{[1
]}

Shibata, Masatoshi ^{[1
]}

Tsuchiya, Seiko ^{[1
]}

Enosawa, Shin ^{[4
]}

Takeuchi, Koichi ^{[3
]}

Konno, Kenjiro ^{[5
]}

Hakamata, Yoji ^{[5
]}

Yoshino, Hiroyuki ^{[6
]}

Wakai, Takuya ^{[6
]}

Ookawara, Shigeo ^{[3
]}

Tanaka, Hozumi ^{[5
]}

Kobayashi, Eiji ^{[6
]}

Murakami, Takashi ^{[6
]}

机构：

[1] Shizuoka Prefectural Livestock Inst, Swine & Poultry Res Ctr, Shizuoka 4390037, Japan

[2] Kitasato Univ, Sch Med, Dept Lab Anim Sci, Kanagawa 2288555, Japan

[3] Jichi Med Univ, Div Anat, Dept Anat, Shimotsuke, Tochigi 3290498, Japan

[4] Natl Res Inst Child Hlth & Dev, Dept Innovat Surg, Tokyo 1578535, Japan

[5] Jichi Med Univ, Ctr Med Expt, Shimotsuke, Tochigi 3290498, Japan

[6] Jichi Med Univ, Ctr Mol Med, Div Organ Replacement Res, Shimotsuke, Tochigi 3290498, Japan

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2009年 / 14卷 / 05期

基金：

日本学术振兴会;

关键词：

Jinhua pigs; green fluorescent protein; biological probes; somatic cell cloning; RATS; EMBRYOS; EXPRESSION;

D O I：

10.1117/1.3241985

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Animal imaging sources have become an indispensable material for biological sciences. Specifically, gene-encoded biological probes serve as stable and high-performance tools to visualize cellular fate in living animals. We use a somatic cell cloning technique to create new green fluorescent protein (GFP)-expressing Jinhua pigs with a miniature body size, and characterized the expression profile in various tissues/organs and ex vivo culture conditions. The born GFP-transgenic pig demonstrate an organ/tissue-dependent expression pattern. Strong GFP expression is observed in the skeletal muscle, pancreas, heart, and kidney. Regarding cellular levels, bone-marrow-derived mesenchymal stromal cells, hepatocytes, and islet cells of the pancreas also show sufficient expression with the unique pattern. Moreover, the cloned pigs demonstrate normal growth and fertility, and the introduced GFP gene is stably transmitted to pigs in subsequent generations. The new GFP-expressing Jinhua pigs may be used as new cellular/tissue light resources for biological imaging in preclinical research fields such as tissue engineering, experimental regenerative medicine, and transplantation. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3241985]

引用

页数：9

共 33 条

[11] Co-transfer of parthenogenotes and single porcine embryos leads to full-term development of the embryos
Kawarasaki, T.
Otake, M.
Tsuchiya, S.
Shibata, M.
Matsumoto, K.
Isobe, N.
[J]. ANIMAL REPRODUCTION SCIENCE, 2009, 112 (1-2) : 8 - 21
[12] Production of transgenic-clone pigs by the combination of ICSI-mediated gene transfer with somatic cell nuclear transfer
Kurome, M
Ueda, H
Tomii, R
Naruse, K
Nagashima, H
[J]. TRANSGENIC RESEARCH, 2006, 15 (02) : 229 - 240
[13] Li SJ, 2004, J ANIM SCI, V82, P368
[14] Development and use of fluorescent protein markers in living cells
Lippincott-Schwartz, J
Patterson, GH
[J]. SCIENCE, 2003, 300 (5616) : 87 - 91
[15] Analysis of green fluorescent protein expression in transgenic rats for tracking transplanted neural stem/progenitor cells
Mothe, AJ
Kulbatski, I
van Bendegem, RL
Lee, L
Kobayashi, E
Keating, A
Tator, CH
[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2005, 53 (10) : 1215 - 1226
[16] Color-engineered rats and luminescent LacZ imaging: a new platform to visualize biological processes
Murakami, T
Kobayashi, E
[J]. JOURNAL OF BIOMEDICAL OPTICS, 2005, 10 (04)
[17] FISH analysis of 142 EGFP transgene integration sites into the mouse genome
Nakanishi, T
Kuroiwa, A
Yamada, S
Isotani, A
Yamashita, A
Tairaka, A
Hayashi, T
Takagi, T
Ikawa, M
Matsuda, Y
Okabe, M
[J]. GENOMICS, 2002, 80 (06) : 564 - 574
[18] Epigenetic reprogramming in embryonic and foetal development upon somatic cell nuclear transfer cloning
Niemann, Heiner
Tian, X. Cindy
King, W. Allan
Lee, Rita S. F.
[J]. REPRODUCTION, 2008, 135 (02) : 151 - 163
[19] NIWA H, 1991, GENE, V108, P193, DOI 10.1016/0378-1119(91)90434-D
[20] Early death of mice cloned from somatic cells
Ogonuki, N
Inoue, K
Yamamoto, Y
Noguchi, Y
Tanemura, K
Suzuki, O
Nakayama, H
Doi, K
Ohtomo, Y
Satoh, M
Nishida, A
Ogura, A
[J]. NATURE GENETICS, 2002, 30 (03) : 253 - 254

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