Increase of Regeneration Efficiency in JATROPHA CURCAS by In Vitro Culture Methods

An effective protocol has been established to induce adventitious buds regeneration from 3 types of explants (petiole, petiolule, cotyledon petiole) of Jatropha curcas L. All the explants were dealt with high concentrations (5-120 mg/L) of TDZ solution for various soak time (5-80 min) with the regeneration frequency increased and the quality of the regenerated shoot-buds improved significantly compared with the traditional regeneration approach. The best result of adventitious buds regeneration was observed when explants were treated with 20 mg/L TDZ solution for 20 min before being placed on hormone-free MS medium for 30 days of culture. Moreover, addition of GA3 to the elongation medium was contributed to the prolongation of the shoot buds and the best elongate results could be gained at a concentration of 0.4 mg/L. By application of the new protocol described in this paper, plant regeneration efficiency of J. curcas was increased observably and the culture period was shortened in 65 days.