The removal of fluorescence in sequencing-by-synthesis

被引：10
作者：
Gao, Li [1 ]
Lu, Zuhong [1 ,2 ]
机构：
[1] Southeast Univ, State Key Lab Bioelect, Sch Biol Sci & Med Engn, Nanjing 210096, Peoples R China
[2] Southeast Univ, Minist Educ China, Key Lab Child Dev & Learning Sci, Nanjing 210096, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 387卷 / 03期
关键词：
DNA sequencing; Fluorescence removal; Sequencing-by-synthesis; NUCLEOTIDE REVERSIBLE TERMINATORS; PRIMER EXTENSION; DNA; DESIGN; POLYMORPHISMS; CHEMISTRY;
D O I：
10.1016/j.bbrc.2009.07.068
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Fluorescent oligonucleotides provide useful Way to Study DNA during the sequencing-by-synthesis process. They allow researchers to determine the sequence of a short DNA strand. Fluorescence created by these chemicals must be removed after a few based pairs are read in order to Continue reading the DNA sequence. Researchers have developed a variety of methods to solve this problem. This paper reviews the development of fluorescence removal in DNA sequencing and summarizes several methods of removing the fluorescence. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码：421 / 424
页数：4
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