Polyelectrolyte microcapsules and coated CaCO3 particles as fluorescence activated sensors in flowmetry

被引:33
作者
Halozan, David [1 ]
Riebentanz, Uta [2 ]
Brumen, Milan [3 ,4 ]
Donath, Edwin [2 ]
机构
[1] Inst Phys Biol, Ljubljana 1000, Slovenia
[2] Univ Leipzig, Inst Med Phys & Biophys, D-04107 Leipzig, Germany
[3] Jozef Stefan Inst, Ljubljana 1000, Slovenia
[4] Univ Maribor, Fac Med, SLO-2000 Maribor, Slovenia
关键词
Calcium carbonate particles; Polyelectrolyte microcapsules; pH sensor; Salt sensor; Flowmetry; X-RAY MICROSCOPY; MULTILAYER MICROCAPSULES; PROTEIN ENCAPSULATION; DONNAN EQUILIBRIUM; THERMAL-BEHAVIOR; FLOW-CYTOMETRY; LIVING CELLS; CAPSULES; MICROPARTICLES; NANOSENSORS;
D O I
10.1016/j.colsurfa.2009.04.024
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Polyelectrolyte multilayer film capsules with different micrometer sizes were assembled for pH and salt sensing devices. Encapsulation of labelled poly(acrylic acid) (PAA(AF)) in CaCO3 particles increases their potential usage in the biological environment. Microcapsules were analyzed with flowcytometry to obtain individual response of a spherical-like microsensor that behaves as Donnan system. The linear pH response of microparticles and microcapsules is quite broad and is useful in physiological range. The salt response is up to 0.1 M and was also predicted with simple mathematical model. (c) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:115 / 121
页数:7
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