Induced Pluripotent Stem Cells and Embryonic Stem Cells Are Distinguished by Gene Expression Signatures

被引：770

作者：

Chin, Mark H. ^{[1
]}

Mason, Mike J. ^{[1
,9
]}

Xie, Wei ^{[1
]}

Volinia, Stefano ^{[11
]}

Singer, Mike ^{[12
]}

Peterson, Cory ^{[3
]}

Ambartsumyan, Gayane ^{[2
]}

Aimiuwu, Otaren ^{[2
]}

Richter, Laura ^{[2
]}

Zhang, Jin ^{[4
,5
]}

Khvorostov, Ivan ^{[4
,5
]}

Ott, Vanessa ^{[12
]}

Grunstein, Michael ^{[1
]}

Lavon, Neta ^{[10
]}

Benvenisty, Nissim ^{[10
]}

Croce, Carlo M. ^{[11
]}

Clark, Amander T. ^{[2
,6
,7
,8
]}

Baxter, Tim ^{[12
]}

Pyle, April D. ^{[3
,6
,7
,8
]}

Teitell, Mike A. ^{[4
,5
,6
,7
,8
]}

Pelegrini, Matteo ^{[2
,8
]}

Plath, Kathrin ^{[1
,6
,7
,8
]}

Lowry, William E. ^{[2
,6
,7
,8
]}

机构：

[1] Univ Calif Los Angeles, David Geffen Sch Med, Dept Biol Chem, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA

[4] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pathol, Los Angeles, CA 90095 USA

[5] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pediat, Los Angeles, CA 90095 USA

[6] Univ Calif Los Angeles, Broad Stem Cell Ctr, Los Angeles, CA 90095 USA

[7] Univ Calif Los Angeles, Jonsson Comprehens Canc Ctr, Los Angeles, CA 90095 USA

[8] Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90095 USA

[9] Univ Calif Los Angeles, Dept Stat, Los Angeles, CA 90095 USA

[10] Hebrew Univ Jerusalem, Dept Genet, IL-91904 Jerusalem, Israel

[11] Ohio State Univ, Dept Mol Virol Immunol & Med Genet, Columbus, OH 43210 USA

[12] Roche NimbleGen Inc, Madison, WI 53719 USA

来源：

CELL STEM CELL | 2009年 / 5卷 / 01期

关键词：

DIFFERENTIATION; FIBROBLASTS; GENERATION; INDUCTION; SKIN; DERIVATION;

D O I：

10.1016/j.stem.2009.06.008

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Induced pluripotent stem cells (iPSCs) outwardly appear to be indistinguishable from embryonic stem cells (ESCs). A study of gene expression profiles of mouse and human ESCs and iPSCs suggests that, while iPSCs are quite similar to their embryonic counterparts, a recurrent gene expression signature appears in iPSCs regardless of their origin or the method by which they were generated. Upon extended culture, hiPSCs adopt a gene expression profile more similar to hESCs; however, they still retain a gene expression signature unique from hESCs that extends to miRNA expression. Genome-wide data suggested that the iPSC signature gene expression differences are due to differential promoter binding by the reprogramming factors. High-resolution array profiling demonstrated that there is no common specific subkaryotypic alteration that is required for reprogramming and that reprogramming does not lead to genomic instability. Together, these data suggest that iPSCs should be considered a unique subtype of pluripotent cell.

引用

页码：111 / 123

页数：13

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