Structure of toroidal DNA collapsed inside the phage capsid

被引：109

作者：

Leforestier, Amelie ^{[1
]}

Livolant, Francoise ^{[1
]}

机构：

[1] Univ Paris 11, CNRS, Phys Solides Lab, Ctr Orsay,UMR 8502, F-91405 Orsay, France

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 23期

关键词：

bacteriophage capsid; DNA helical pitch; DNA correlation; DNA toroid; cryoelectron microscopy; ITERATED MOIRE MAPS; COMPACT FORM; EJECTION; CONDENSATION; PRESSURE; PHASES; MICROSCOPY; SPERMIDINE; RESOLUTION; CURVATURE;

D O I：

10.1073/pnas.0901240106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The structure of DNA toroids made of individual DNA molecules of various lengths (3,000 to 55,000 bp) was studied, by using partially filled bacteriophage capsids in conjunction with cryoelectron microscopy. The tetravalent cation spermine was diffused through the capsid to condense the DNA under conditions that were chosen to produce a hexagonal packing. Our results demonstrate that the frustration arising between chirality and hexagonal packing leads to the formation of twist walls; the correlation between helices combined with their strong curvature impose variations of the DNA helical pitch.

引用

页码：9157 / 9162

页数：6

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