Intradermal delivery of receptor-binding domain of SARS-CoV-2 spike protein with dissolvable microneedles to induce humoral and cellular responses in mice

被引：37
作者：
Kuwentrai, Chaiyaporn [1 ]
Yu, Jinming [2 ]
Rong, Li [1 ]
Zhang, Bao-Zhong [1 ,3 ]
Hu, Ye-Fan [1 ,4 ]
Gong, Hua-Rui [1 ]
Dou, Ying [1 ]
Deng, Jian [1 ]
Huang, Jian-Dong [1 ,3 ]
Xu, Chenjie [2 ]
机构：
[1] Univ Hong Kong, Li Ka Shing Fac Med, Sch Biomed Sci, 21 Sassoon Rd, Hong Kong, Peoples R China
[2] City Univ Hong Kong, Dept Biomed Engn, Hong Kong, Peoples R China
[3] Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Synthet Biol, Shenzhen, Peoples R China
[4] Univ Hong Kong, Queen Mary Hosp, Dept Med, Hong Kong, Peoples R China
来源：
BIOENGINEERING & TRANSLATIONAL MEDICINE | 2021年 / 6卷 / 01期
基金：
中国博士后科学基金;
关键词：
COVID-19; intradermal delivery; microneedle; receptor-binding domain; SARS-CoV-2; spike protein;
D O I：
10.1002/btm2.10202
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The S1 subunit of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein contains an immunogenic receptor-binding domain (RBD), which is a promising candidate for the development of a potential vaccine. This study demonstrated that intradermal delivery of an S-RBD vaccine using a dissolvable microneedle skin patch can induce both significant B-cell and significant T-cell responses against S-RBD. Importantly, the outcomes were comparable to that of conventional bolus injection.
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页数：9
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