Deregulation of Bmi-1 is associated with enhanced migration, invasion and poor prognosis in salivary adenoid cystic carcinoma

被引：27

作者：

Chang, Boyang ^{[1
,2
]}

Li, Su ^{[1
]}

He, Qianting ^{[2
]}

Liu, Zhonghua ^{[2
]}

Zhao, Luodan ^{[2
]}

Zhao, Tinging ^{[2
]}

Wang, Anxun ^{[2
]}

机构：

[1] Sun Yat Sen Univ, Ctr Canc, State Key Lab Oncol Southern China, Guangzhou 510060, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Oral & Maxillofacial Surg, Guangzhou 510080, Guangdong, Peoples R China

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2014年 / 1840卷 / 12期

关键词：

Bmi-1; Salivary adenoid cystic carcinoma; Metastasis; EPITHELIAL-MESENCHYMAL TRANSITION; STEM-CELLS; PROMOTES MIGRATION; SELF-RENEWAL; IN-VITRO; EXPRESSION; CANCER; METASTASIS; ERK; PROLIFERATION;

D O I：

10.1016/j.bbagen.2014.08.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Bmi-1 had been found to involve in self renewal of stem cells and tumorigenesis in various malignancies. In this study, we investigated the role of Bmi-1 in the development of salivary adenoid cystic carcinoma (SACC). Methods: At first, we confirmed that the deregulation of Bmi-1 was a frequent event in SACC; up-regulation of Bmi-1 was correlated with clinical stages, vital status and distant metastasis and associated with reduced overall survival and disease free survival. SACC-LM cells, higher migration and invasion abilities, elevated the expression of Bmi-1 protein, epithelial-mesenchymal transition (EMT) related proteins (Snail, Slug and Vimentin) and cancer stem cells (CSCs) related proteins (ABCG(2), Notch, ALDH-1, Oct-4, Nanog and Epcam) compared to the SACC-83 cells (lower migration and invasion abilities). The migration and invasion abilities were inhibited in SACC-LM cells upon Bmi-1 knockdown. Meanwhile, Bmi-1 knockdown resulted in simultaneous loss of stem cell markers and EMT markers in SACC-LM cells. Conclusion: Our studies confirm that Bmi-1 deregulation plays an important role in the development of SACC and contributes to the migration and the invasion abilities of SACC, which is involved in EMT and CSCs. General significance: To our knowledge, this is the first study revealing that Bmi-1 deregulation is associated with enhanced migration, invasion and poor prognosis in salivary adenoid cystic carcinoma. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：3285 / 3291

页数：7

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