Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates

被引：33

作者：

Dorneles, Elaine M. S. ^{[1
]}

Santana, Jordana A. ^{[1
]}

Ribeiro, Dayana ^{[2
]}

Dorella, Fernanda Alves ^{[2
]}

Guimaraes, Alessandro S. ^{[3
,4
]}

Moawad, Mohamed S. ^{[5
]}

Selim, Salah A. ^{[5
]}

Garaldi, Ana Luiza M. ^{[6
]}

Miyoshi, Anderson ^{[2
]}

Ribeiro, Marcio G. ^{[7
]}

Gouveia, Aurora M. G. ^{[1
]}

Azevedo, Vasco ^{[2
]}

Heinemann, Marcos B. ^{[1
]}

Lage, Andrey P. ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Dept Med Vet Prevent, Escola Vet, Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Biol Geral, Belo Horizonte, MG, Brazil

[3] Empresa Brasileira Pesquisa Agr, Embrapa Gado Leite, Juiz De Fora, Brazil

[4] Univ Fed Lavras, Dept Vet Med, Lavras, MG, Brazil

[5] Cairo Univ, Dept Toxicol & Forens Med, Cairo, Egypt

[6] Univ Estado Rio de Janeiro, Ctr Biomed, Fac Ciencias Med, BR-20550011 Rio De Janeiro, Brazil

[7] Univ Estadual Paulista, Dept Higiene Vet & Saude Publ, Fac Med Vet & Zootecnia, Botucatu, SP, Brazil

来源：

PLOS ONE | 2014年 / 9卷 / 06期

关键词：

COMPLETE GENOME SEQUENCE; CASEOUS-LYMPHADENITIS; MOLECULAR CHARACTERIZATION; NECROTIZING LYMPHADENITIS; TYPING SYSTEMS; SHEEP; INFECTION; PREVALENCE; SAMPLES; CATTLE;

D O I：

10.1371/journal.pone.0098758

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410(T)) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.

引用

页数：10

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