Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak

被引：20

作者：

Correia, Barbara ^{[1
,2
]}

Luis Rodriguez, Jose ^{[3
]}

Valledor, Luis ^{[4
]}

Almeida, Tania ^{[5
,6
]}

Santos, Conceicao ^{[1
,2
]}

Jesus Canal, Maria ^{[3
]}

Pinto, Gloria ^{[1
,2
]}

机构：

[1] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal

[2] Univ Aveiro, CESAM Ctr Environm & Marine Studies, P-3810193 Aveiro, Portugal

[3] Univ Oviedo, Univ Inst Biotechnol Asturias IUBA, BOS Dept, EPIPHYSAGE Res Grp, Oviedo, Spain

[4] Acad Sci Czech Republ, Global Change Res Ctr, Brno 60300, Czech Republic

[5] Ctr Biotecnol Agr & Agroalimentar Alentejo CEBAL, Inst Politecn Beja IPBeja, P-7801908 Beja, Portugal

[6] Univ Aveiro, Ctr Res Ceram & Composite Mat CICECO, P-3810193 Aveiro, Portugal

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2014年 / 171卷 / 06期

关键词：

High temperature; Plant performance; Quercus suber; Recovery; QUERCUS-SUBER; HIGH-TEMPERATURE; LIPID-PEROXIDATION; PHENOTYPIC PLASTICITY; CHALCONE SYNTHASE; DIURNAL CHANGES; SHOCK PROTEINS; PHOTOSYNTHESIS; RESPONSES; WATER;

D O I：

10.1016/j.jplph.2013.12.004

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cork oak (Quercus suber L.) is a research priority in the Mediterranean area and because of cork oaks' distribution these stands are experiencing daily stress. Based on projections of intensifying climate change and considering the key role of exploring the recovery abilities, cork oak seedlings were subjected to a cumulative temperature increase from 25 degrees C to 55 degrees C and subsequent recovery. CO2 assimilation rate, chlorophyll fluorescence, anthocyanins, proline and lipid peroxidation were used to evaluate plant performance, while the relative abundance of seven genes encoding for proteins of cork oak with a putative role in thermal/stress regulation (POX1, POX2, HSP10.4, HSP17a.22, CHS, MTL and RBC) was analyzed by qPCR (quantitative Polymerase Chain Reaction). A temperature change to 35 degrees C showed abundance alterations in the tested genes; at 45 degrees C, the molecular changes were associated with an antioxidant response, possibly modulated by anthocyanins. At 55 degrees C, HSP17a.22, MTL and proline accumulation were evident. After recovery, physiological balance was restored, whereas POX1, HSP10.4 and MTL abundances were suggested to be involved in increased thermotolerance. The data presented here are expected to pinpoint some pathways changes occurring during such stress and further recovery in this particular Mediterranean species. (C) 2013 Elsevier GmbH. All rights reserved.

引用

页码：399 / 406

页数：8

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