Role of IL-10 Deficiency in Pneumonia Induced by Corynebacterium kutscheri in Mice

被引:11
作者
Jeong, Eui-Suk [1 ,2 ]
Won, Young-Suk [3 ]
Kim, Hyoung-Chin [3 ]
Cho, Myung-Hawn [2 ]
Choi, Yang-Kyu [1 ]
机构
[1] Konkuk Univ, Coll Vet Med, Seoul 143701, South Korea
[2] Konkuk Univ, Coll Sci, Dept Biol Sci, Seoul 143701, South Korea
[3] Korea Res Inst Biosci & Biotechnol, Bioevaluat Ctr, Cheongwon 363883, Chungbuk, South Korea
关键词
Corynebacterium kutscheri; IL-10; KO; IFN-gamma; MIP-1; alpha; IP-10; GAMMA-INTERFERON; PSEUDOMONAS-AERUGINOSA; CYSTIC-FIBROSIS; HYPERSENSITIVITY PNEUMONITIS; INFLAMMATORY RESPONSE; LUNG INFECTION; KNOCKOUT MICE; INTERLEUKIN-10; EXPRESSION; CELLS;
D O I
10.4014/jmb.0807.436
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
IL-10 is an important anti-inflammatory cytokine that can inhibit the production of many pro-inflammatory cytokines. Both human and animal studies have shown that pro-inflammatory cytokines play an important role in pneumonia and other inflammatory lung diseases. In the present study, IL-10 knockout (KO) and wild-type mice were infected with Corynebacterium kutscheri to determine whether the severity of pathogenesis and whether protective immunity could be altered in the absence of IL-10. The survival rate was significantly lower in IL-10 KO mice than wild-type mice. The number of neutrophils in bronchoalveolar lavage fluid and blood were found to be higher in IL-10 KO mice than wild-type mice. IL-10 KO mice showed greater neutrophil infiltration, excessive inflammation, and weight-loss compared with wild-type mice. Furthermore, upregulation of IFN-gamma in bronchoalveolar lavage fluid, and upregulation of MIP-1 alpha and IP-10 mRNA in the lungs of IL-10 KO mice compared with wild-type mice after C kutscheri infection were observed. These results suggest that IL-10 plays an important role in the anti-inflammatory properties against C kutscheri infection, and that lack of IL-10 leads to a more severe pulmonary inflammatory response. This increased susceptibility to C kutscheri pneumonia is at least in part caused by IL-10 deficiency and severe recruitment of neutrophils.
引用
收藏
页码:424 / 430
页数:7
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