Effect of selenium-sulfur interaction on the anabolism of sulforaphane in broccoli

被引：18

作者：

Mao, Shuxiang ^{[1
,2
,3
]}

Wang, Junwei ^{[1
,2
,3
]}

Wu, Qi ^{[1
,2
,3
]}

Liang, Mantian ^{[1
,2
,3
]}

Yuan, Yiming ^{[1
,2
,3
]}

Wu, Tao ^{[1
,2
,3
]}

Liu, Mingyue ^{[1
,2
,3
]}

Wu, Qiuyun ^{[1
,2
,3
]}

Huang, Ke ^{[1
,2
,3
]}

机构：

[1] Hunan Agr Univ, Coll Hort, Changsha 410128, Peoples R China

[2] Engn Res Ctr Hort Crop Germplasm Creat & New Vari, Minist Educ, Changsha 410128, Peoples R China

[3] Key Lab Vegetable Biol Hunan Prov, Changsha 410128, Peoples R China

来源：

PHYTOCHEMISTRY | 2020年 / 179卷

基金：

中国国家自然科学基金;

关键词：

Brassica oleracea L. var. italica; Selenium; Biofortified; Bioactive substance; Sulforaphane; Glucosinolate; Myrosinase; AMINO-ACID; GLUCOSINOLATE CONCENTRATION; ARABIDOPSIS-THALIANA; BRASSICA; BIOSYNTHESIS; FERTILIZATION; ACCUMULATION; METABOLITES; MYROSINASE; EXPRESSION;

D O I：

10.1016/j.phytochem.2020.112499

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of S (as sulphate) and Se (as selenite) treatment (S mM/Se mu M: 1/0, 1/50, 1/100, 1/150, 4/0, 4/50, 4/100, and 4/150) on the production of sulforaphane (an anticancer compound), the accumulation of its precursor substance, and the expression of genes related to glucoraphanin biosynthesis in broccoli were examined. Sulforaphane yield and myrosinase activity increased significantly with the combined application of 4 mM S and 100 mu M Se on broccoli. Furthermore, the concentrations of glucoraphanin (a sulforaphane precursor) and methionine (a glucoraphanin substrate) slightly changed after Se application. And the strong anticancer activity of compound Se-SMC was further improved. Analysis of related gene expression showed that MY, which encodes myrosinase, was strongly induced by Se treatment. Thus, the myrosinase activity induced by Se treatment is the dominant factor affecting sulforaphane yield from glucoraphanin hydrolyzation. Selenium-sulfur biofortification provides a technical support for the cultivation of broccoli with high sulforaphane and high anti-cancer selenium compounds.

引用

页数：9

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