Murine Stat2 is uncharacteristically divergent

被引:57
作者
Park, C
Lecomte, MJ
Schindler, C
机构
[1] Columbia Univ, Dept Microbiol, New York, NY 10032 USA
[2] Columbia Univ, Dept Med, New York, NY 10032 USA
关键词
D O I
10.1093/nar/27.21.4191
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Characterization of the ability of human interferons (IFNs) to rapidly induce genes led to the identification of the first two members of the STAT (signal transducers and activators of transcription) family, Stat1 and Stat2, To study the unique role of this transcription factor in IFN signaling under more physiological conditions, murine Stat2 was isolated and found to be surprisingly divergent. This divergence was most striking in the C-terminal transcriptional activation domain. Studies on murine Stat2 indicate that it functions in IFN signaling. This includes IFN-alpha-dependent activation, nuclear translocation, DNA binding and activation of reporter genes. However, the profound divergence at the C-terminus suggests that murine Stat2 may have evolved to mediate some unique functions as well. To explore this possibility, proteins that interact with the C-termini of murine and human Stat2 were examined. These studies indicate that the murine and human C-termini interact with an overlapping, but distinct set of proteins.
引用
收藏
页码:4191 / 4199
页数:9
相关论文
共 52 条
[1]   Functionally distinct isoforms of STAT5 are generated by protein processing [J].
Azam, M ;
Lee, C ;
Strehlow, I ;
Schindler, C .
IMMUNITY, 1997, 6 (06) :691-701
[2]   INTERLEUKIN-3 SIGNALS THROUGH MULTIPLE ISOFORMS OF STAT5 [J].
AZAM, M ;
ERDJUMENTBROMAGE, H ;
KREIDER, BL ;
XIA, M ;
QUELLE, F ;
BASU, R ;
SARIS, C ;
TEMPST, P ;
IHLE, JN ;
SCHINDLER, C .
EMBO JOURNAL, 1995, 14 (07) :1402-1411
[3]   Three-dimensional structure of the Stat3β homodimer bound to DNA [J].
Becker, S ;
Groner, B ;
Müller, CW .
NATURE, 1998, 394 (6689) :145-151
[4]   Cooperation of Stat2 and p300/CBP in signalling induced by interferon-alpha [J].
Bhattacharya, S ;
Eckner, R ;
Grossman, S ;
Oldread, E ;
Arany, Z ;
DAndrea, A ;
Livingston, DM .
NATURE, 1996, 383 (6598) :344-347
[5]   Stat2 is a transcriptional activator that requires sequence-specific contacts provided by Stat1 and p48 for stable interaction with DNA [J].
Bluyssen, HAR ;
Levy, DE .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (07) :4600-4605
[6]  
BROEK MFV, 1995, J VIROL, V69, P4792
[7]   STAT3 beta, a splice variant of transcription factor STAT3, is a dominant negative regulator of transcription [J].
Caldenhoven, E ;
vanDijk, TB ;
Solari, R ;
Armstrong, J ;
Raaijmakers, JAM ;
Lammers, JWJ ;
Koenderman, L ;
deGroot, RP .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (22) :13221-13227
[8]   Crystal structure of a tyrosine phosphorylated STAT-1 dimer bound to DNA [J].
Chen, XM ;
Vinkemeier, U ;
Zhao, YX ;
Jeruzalmi, D ;
Darnell, JE ;
Kuriyan, J .
CELL, 1998, 93 (05) :827-839
[9]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[10]   DISTRIBUTION OF THE MAMMALIAN STAT GENE FAMILY IN MOUSE CHROMOSOMES [J].
COPELAND, NG ;
GILBERT, DJ ;
SCHINDLER, C ;
ZHONG, Z ;
WEN, Z ;
DARNELL, JE ;
MUI, ALF ;
MIYAJIMA, A ;
QUELLE, FW ;
IHLE, JN ;
JENKINS, NA .
GENOMICS, 1995, 29 (01) :225-228