A ThCAP gene from Tamarix hispida confers cold tolerance in transgenic Populus (P-davidiana x P-bolleana)

被引：19

作者：

Guo, Xiao-Hong ^{[1
]}

Jiang, Jing ^{[1
]}

Lin, Shi-Jie ^{[1
]}

Wang, Bai-Chen ^{[1
]}

Wang, Yu-Cheng ^{[1
]}

Liu, Gui-Feng ^{[1
]}

Yang, Chuan-Ping ^{[1
]}

机构：

[1] NE Forestry Univ, Key Lab Forest Tree Genet Improvement & Biotechno, Minist Educ, Harbin 150040, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2009年 / 31卷 / 07期

关键词：

Cold acclimation protein; Cold tolerance; Functional analysis; Populus; Tamarix hispida; ABIOTIC STRESS; ARABIDOPSIS; ENHANCEMENT; PROTEINS; DROUGHT; IDENTIFICATION; ACCLIMATION; SALINITY; RNA;

D O I：

10.1007/s10529-009-9959-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The ThCAP gene, which encodes a cold acclimation protein, was isolated from a Tamarix hispida NaCl-stress root cDNA library; its expression patterns were then assayed by qRT-PCR in different T. hispida tissues treated with low temperature (4A degrees C), salt (400 mM NaCl), drought (20% PEG6000) and exogenous abscisic acid (100 mu M). Induction of ThCAP gene was not only responsive to different stress conditions but was also organ specific. When transgenic Populus (P. davidiana x P. bolleana) plants were generated, expressing ThCAP under regulation of the cauliflower mosaic virus CaMV 35S promoter, they had a greater resistance to low temperature than non-transgenic seedlings, suggesting that ThCAP might play an important role in cold tolerance.

引用

页码：1079 / 1087

页数：9

共 26 条

[1] Expression profiling and bioinformatic analyses of a novel stress-regulated multispanning transmembrane protein family from cereals and Arabidopsis
Breton, G
Danyluk, J
Charron, JBF
Sarhan, F
[J]. PLANT PHYSIOLOGY, 2003, 132 (01) : 64 - 74
[2] Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes
Chen, THH
Murata, N
[J]. CURRENT OPINION IN PLANT BIOLOGY, 2002, 5 (03) : 250 - 257
[3] Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana
Ferro, M
Salvi, D
Brugière, S
Miras, S
Kowalski, S
Louwagie, M
Garin, J
Joyard, J
Rolland, N
[J]. MOLECULAR & CELLULAR PROTEOMICS, 2003, 2 (05) : 325 - 345
[4] Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway
Fowler, S
Thomashow, MF
[J]. PLANT CELL, 2002, 14 (08) : 1675 - 1690
[5] In-depth analysis of the thylakoid membrane proteome of Arabidopsis thaliana chloroplasts:: New proteins, new functions, and a plastid proteome database
Friso, G
Giacomelli, L
Ytterberg, AJ
Peltier, JB
Rudella, A
Sun, Q
van Wijk, KJ
[J]. PLANT CELL, 2004, 16 (02) : 478 - 499
[6] Proteomic study of the Arabidopsis thaliana chloroplastic envelope membrane utilizing alternatives to traditional two-dimensional electrophoresis
Froehlich, JE
Wilkerson, CG
Ray, WK
McAndrew, RS
Osteryoung, KW
Gage, DA
Phinney, BS
[J]. JOURNAL OF PROTEOME RESEARCH, 2003, 2 (04) : 413 - 425
[7] An Arabidopsis mutation in translation elongation factor 2 causes superinduction of CBF/DREB1 transcription factor genes but blocks the induction of their downstream targets under low temperatures
Guo, Y
Xiong, L
Ishitani, M
Zhu, JK
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (11) : 7786 - 7791
[8] Enhancement of spermidine content and antioxidant capacity in transgenic pear shoots overexpressing apple spermidine synthase in response to salinity and hyperosmosis
He, Lixiong
Ban, Yusuke
Inoue, Hiromichi
Matsuda, Narumi
Liu, Jihong
Moriguchi, Takaya
[J]. PHYTOCHEMISTRY, 2008, 69 (11) : 2133 - 2141
[9] Transgenic tobacco plants overproducing alfalfa aldose/aldehyde reductase show higher tolerance to low temperature and cadmium stress
Hegedüs, A
Erdei, S
Janda, T
Tóth, E
Horváth, G
Dudits, D
[J]. PLANT SCIENCE, 2004, 166 (05) : 1329 - 1333
[10] Isolation of high quality RNA from bilberry (Vaccinium myrtillus L.) fruit
Jaakola, L
Pirttilä, AM
Halonen, M
Hohtola, A
[J]. MOLECULAR BIOTECHNOLOGY, 2001, 19 (02) : 201 - 203

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