Further evidence to support the melanocytic origin of MDA-MB-435

被引：137

作者：

Ellison, G ^{[1
]}

Klinowska, T ^{[1
]}

Westwood, RFR ^{[1
]}

Docter, E ^{[1
]}

French, T ^{[1
]}

Fox, JC ^{[1
]}

机构：

[1] AstraZeneca, Macclesfield SK10 4TG, Cheshire, England

来源：

JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY | 2002年 / 55卷 / 05期

关键词：

D O I：

10.1136/mp.55.5.294

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Background/Aims: Until recently, the cell line MDA-MB-435 was widely accepted as originating from a breast cancer. However, microarray derived data have suggested that this cell line may in fact originate from an occult melanoma. This study was designed to investigate this hypothesis further. Methods: Quantitative reverse transcription polymerase chain reaction and immunohistochemistry were used to investigate the tissue of origin of two sublines of MDA-MB-435 (MDA-MB-435 S and MDA-MB-435 HGF). The expression of a panel of genes typical of breast cells or melanocytes was analysed. Results: The MDA-MD-435 cell lines expressed none of the genes characteristic of breast cancer cells but did express several genes commonly expressed by melanocytes. Conclusions: These results strongly suggest that MDA-MB-435 is indeed of melanoma origin.

引用

页码：294 / 299

页数：6

共 18 条

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